7WTW

Cryo-EM structure of a human pre-40S ribosomal subunit - State RRP12-A3

7WTW の概要

• エントリーDOI: 10.2210/pdb7wtw/pdb
• EMDBエントリー: 32803
• 分子名称: 18S rRNA, 40S ribosomal protein S6, 40S ribosomal protein S25, ... (38 entities in total)
• 機能のキーワード: ribosome biogenesis, 40s ribosome, ribosome
• 由来する生物種: Homo sapiens; 詳細
• タンパク質・核酸の鎖数: 37
• 化学式量合計: 1587358.57

構造登録者

Cheng, J.,Lau, B.,Thoms, M.,Ameismeier, M.,Berninghausen, O.,Hurt, E.,Beckmann, R. (登録日: 2022-02-05, 公開日: 2022-10-19, 最終更新日: 2024-06-26)

主引用文献

Cheng, J.,Lau, B.,Thoms, M.,Ameismeier, M.,Berninghausen, O.,Hurt, E.,Beckmann, R.
The nucleoplasmic phase of pre-40S formation prior to nuclear export.
Nucleic Acids Res., 50:11924-11937, 2022

PubMed Abstract: Biogenesis of the small ribosomal subunit in eukaryotes starts in the nucleolus with the formation of a 90S precursor and ends in the cytoplasm. Here, we elucidate the enigmatic structural transitions of assembly intermediates from human and yeast cells during the nucleoplasmic maturation phase. After dissociation of all 90S factors, the 40S body adopts a close-to-mature conformation, whereas the 3' major domain, later forming the 40S head, remains entirely immature. A first coordination is facilitated by the assembly factors TSR1 and BUD23-TRMT112, followed by re-positioning of RRP12 that is already recruited early to the 90S for further head rearrangements. Eventually, the uS2 cluster, CK1 (Hrr25 in yeast) and the export factor SLX9 associate with the pre-40S to provide export competence. These exemplary findings reveal the evolutionary conserved mechanism of how yeast and humans assemble the 40S ribosomal subunit, but reveal also a few minor differences.

PubMed: 36321656
DOI: 10.1093/nar/gkac961

実験手法

ELECTRON MICROSCOPY (3.2 Å)