7WSU

Cryo-EM structure of the barley Yellow stripe 1 transporter in complex with Fe(III)-PDMA

7WSU の概要

• エントリーDOI: 10.2210/pdb7wsu/pdb
• EMDBエントリー: 32767
• 分子名称: Iron-phytosiderophore transporter, CHOLESTEROL HEMISUCCINATE, FE (III) ION, ... (4 entities in total)
• 機能のキーワード: iron-phytosiderophore transporter, proline 2'-deoxymugineic acid, metal transport
• 由来する生物種: Hordeum vulgare
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 154837.21

構造登録者

Yamagata, A. (登録日: 2022-02-01, 公開日: 2022-11-30, 最終更新日: 2024-10-23)

主引用文献

Yamagata, A.,Murata, Y.,Namba, K.,Terada, T.,Fukai, S.,Shirouzu, M.
Uptake mechanism of iron-phytosiderophore from the soil based on the structure of yellow stripe transporter.
Nat Commun, 13:7180-7180, 2022

PubMed Abstract: Calcareous soils cover one-third of all land and cause severe growth defects in plants due to the poor water solubility of iron at high pH. Poaceae species use a unique chelation strategy, whereby plants secrete a high-affinity metal chelator, known as phytosiderophores (mugineic acids), and reabsorb the iron-phytosiderophore complex by the yellow stripe 1/yellow stripe 1-like (YS1/YSL) transporter for efficient uptake of iron from the soil. Here, we present three cryo-electron microscopy structures of barley YS1 (HvYS1) in the apo state, in complex with an iron-phytosiderophore complex, Fe(III)-deoxymugineic acid (Fe(III)-DMA), and in complex with the iron-bound synthetic DMA analog (Fe(III)-PDMA). The structures reveal a homodimeric assembly mediated through an anti-parallel β-sheet interaction with cholesterol hemisuccinate. Each protomer adopts an outward open conformation, and Fe(III)-DMA is bound near the extracellular space in the central cavity. Fe(III)-PDMA occupies the same binding site as Fe(III)-DMA, demonstrating that PDMA can function as a potent fertilizer in an essentially identical manner to DMA. Our results provide a structural framework for iron-phytosiderophore recognition and transport by YS1/YSL transporters, which will enable the rational design of new, high-potency fertilizers.

PubMed: 36424382
DOI: 10.1038/s41467-022-34930-1

実験手法

ELECTRON MICROSCOPY (2.9 Å)