7WSJ

Crystal structure of the tandem B-box domain of Arabidopsis thaliana CONSTANS

Summary for 7WSJ

• Entry DOI: 10.2210/pdb7wsj/pdb
• Descriptor: Zinc finger protein CONSTANS, ZINC ION (3 entities in total)
• Functional Keywords: tandem b-box, zn-coordinating motif, photoperiodic flowering, plant protein
• Biological source: Arabidopsis thaliana (thale cress)
• Total number of polymer chains: 3
• Total formula weight: 36822.20

Authors

Dahal, P.,Pathak, D.,Kwon, E.,Kim, D.Y. (deposition date: 2022-01-29, release date: 2022-03-02, Last modification date: 2024-05-29)

Primary citation

Dahal, P.,Kwon, E.,Pathak, D.,Kim, D.Y.
Crystal structure of a tandem B-box domain from Arabidopsis CONSTANS.
Biochem.Biophys.Res.Commun., 599:38-42, 2022

PubMed Abstract: CONSTANS is a central protein in the regulation of photoperiodic flowering, which is expressed in response to day length and promotes the expression of Flowering Locus T. The tandem B-box domain in CONSTANS mediates interactions with various proteins to regulate the expression of Flowering Locus T. Although most plants, including Arabidopsis, have multiple B-box proteins, their B-box structures have not been elucidated. Here, we report the crystal structure of a tandem B-box domain from Arabidopsis CONSTANS. The crystal structure shows that each B-box adopts a canonical B-box fold and coordinates two zinc atoms. Furthermore, the crystal structure reveals that the B-box domain has a unique structure that distinguishes it from animal B-boxes at the monomer and dimer level.

PubMed: 35168062
DOI: 10.1016/j.bbrc.2022.02.025

Experimental method

X-RAY DIFFRACTION (2.4 Å)