7WPF

SARS-CoV-2 Omicron Variant S Trimer complexed with three JMB2002 Fab

7WPF の概要

• エントリーDOI: 10.2210/pdb7wpf/pdb
• EMDBエントリー: 32685
• 分子名称: Spike glycoprotein, JMB2002 Fab heavy chain, JMB2002 Fab light chain, ... (6 entities in total)
• 機能のキーワード: antibody, viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 599914.87

構造登録者

Yin, W.,Xu, Y.,Xu, P.,Cao, X.,Wu, C.,Gu, C.,He, X.,Wang, X.,Huang, S.,Yuan, Q.,Wu, K.,Hu, W.,Huang, Z.,Liu, J.,Wang, Z.,Jia, F.,Xia, K.,Liu, P.,Wang, X.,Song, B.,Zheng, J.,Jiang, H.,Cheng, X.,Jiang, Y.,Deng, S.J.,Xu, H.E. (登録日: 2022-01-23, 公開日: 2022-03-16, 最終更新日: 2024-11-06)

主引用文献

Yin, W.,Xu, Y.,Xu, P.,Cao, X.,Wu, C.,Gu, C.,He, X.,Wang, X.,Huang, S.,Yuan, Q.,Wu, K.,Hu, W.,Huang, Z.,Liu, J.,Wang, Z.,Jia, F.,Xia, K.,Liu, P.,Wang, X.,Song, B.,Zheng, J.,Jiang, H.,Cheng, X.,Jiang, Y.,Deng, S.J.,Xu, H.E.
Structures of the Omicron spike trimer with ACE2 and an anti-Omicron antibody.
Science, 375:1048-1053, 2022

PubMed Abstract: The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant has become the dominant infective strain. We report the structures of the Omicron spike trimer on its own and in complex with angiotensin-converting enzyme 2 (ACE2) or an anti-Omicron antibody. Most Omicron mutations are located on the surface of the spike protein and change binding epitopes to many current antibodies. In the ACE2-binding site, compensating mutations strengthen receptor binding domain (RBD) binding to ACE2. Both the RBD and the apo form of the Omicron spike trimer are thermodynamically unstable. An unusual RBD-RBD interaction in the ACE2-spike complex supports the open conformation and further reinforces ACE2 binding to the spike trimer. A broad-spectrum therapeutic antibody, JMB2002, which has completed a phase 1 clinical trial, maintains neutralizing activity against Omicron. JMB2002 binds to RBD differently from other characterized antibodies and inhibits ACE2 binding.

PubMed: 35133176
DOI: 10.1126/science.abn8863

実験手法

ELECTRON MICROSCOPY (2.92 Å)