7WP6

Cryo-EM structure of SARS-CoV-2 recombinant spike protein STFK in complex with three neutralizing antibodies

7WP6 の概要

• エントリーDOI: 10.2210/pdb7wp6/pdb
• EMDBエントリー: 32676
• 分子名称: 36H6 heavy chain, 83H7 light chain, 83H7 heavy chain, ... (9 entities in total)
• 機能のキーワード: sars-cov-2, spike, vaccine, neutralizing antibody, cryo-em, viral protein, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 96455.18

構造登録者

Zheng, Q.,Sun, H.,Yuan, Q.,Li, S.,Xia, N. (登録日: 2022-01-23, 公開日: 2023-03-01, 最終更新日: 2025-06-18)

主引用文献

Wu, Y.,Wang, S.,Zhang, Y.,Yuan, L.,Zheng, Q.,Wei, M.,Shi, Y.,Wang, Z.,Ma, J.,Wang, K.,Nie, M.,Xiao, J.,Huang, Z.,Chen, P.,Guo, H.,Lan, M.,Xu, J.,Hou, W.,Hong, Y.,Chen, D.,Sun, H.,Xiong, H.,Zhou, M.,Liu, C.,Guo, W.,Guo, H.,Gao, J.,Gan, C.,Li, Z.,Zhang, H.,Wang, X.,Li, S.,Cheng, T.,Zhao, Q.,Chen, Y.,Wu, T.,Zhang, T.,Zhang, J.,Cao, H.,Zhu, H.,Yuan, Q.,Guan, Y.,Xia, N.
Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine.
Cell Host Microbe, 30:1732-1744.e7, 2022

PubMed Abstract: SARS-CoV-2 spread in humans results in continuous emergence of new variants, highlighting the need for vaccines with broad-spectrum antigenic coverage. Using inter-lineage chimera and mutation-patch strategies, we engineered a recombinant monomeric spike variant (STFK1628x) that contains key regions and residues across multiple SAR-CoV-2 variants. STFK1628x demonstrated high immunogenicity and mutually complementary antigenicity to its prototypic form (STFK). In hamsters, a bivalent vaccine composed of STFK and STFK1628x elicited high titers of broad-spectrum neutralizing antibodies to 19 circulating SARS-CoV-2 variants, including Omicron sublineages BA.1, BA.1.1, BA.2, BA.2.12.1, BA.2.75, and BA.4/5. Furthermore, this vaccine conferred robust protection against intranasal challenges by either SARS-CoV-2 ancestral strain or immune-evasive Beta and Omicron BA.1. Strikingly, vaccination with the bivalent vaccine in hamsters effectively blocked within-cage virus transmission of ancestral SARS-CoV-2, Beta variant, and Omicron BA.1 to unvaccinated sentinels. Thus, our study provided insight and antigen candidates for the development of next-generation COVID-19 vaccines.

PubMed: 36323313
DOI: 10.1016/j.chom.2022.10.011

実験手法

ELECTRON MICROSCOPY (3.81 Å)