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7WP6

Cryo-EM structure of SARS-CoV-2 recombinant spike protein STFK in complex with three neutralizing antibodies

7WP6 の概要
エントリーDOI10.2210/pdb7wp6/pdb
EMDBエントリー32676
分子名称36H6 heavy chain, 83H7 light chain, 83H7 heavy chain, ... (9 entities in total)
機能のキーワードsars-cov-2, spike, vaccine, neutralizing antibody, cryo-em, viral protein, immune system-viral protein complex, immune system/viral protein
由来する生物種Severe acute respiratory syndrome coronavirus 2
詳細
タンパク質・核酸の鎖数7
化学式量合計96455.18
構造登録者
Zheng, Q.,Sun, H.,Yuan, Q.,Li, S.,Xia, N. (登録日: 2022-01-23, 公開日: 2023-03-01, 最終更新日: 2025-06-18)
主引用文献Wu, Y.,Wang, S.,Zhang, Y.,Yuan, L.,Zheng, Q.,Wei, M.,Shi, Y.,Wang, Z.,Ma, J.,Wang, K.,Nie, M.,Xiao, J.,Huang, Z.,Chen, P.,Guo, H.,Lan, M.,Xu, J.,Hou, W.,Hong, Y.,Chen, D.,Sun, H.,Xiong, H.,Zhou, M.,Liu, C.,Guo, W.,Guo, H.,Gao, J.,Gan, C.,Li, Z.,Zhang, H.,Wang, X.,Li, S.,Cheng, T.,Zhao, Q.,Chen, Y.,Wu, T.,Zhang, T.,Zhang, J.,Cao, H.,Zhu, H.,Yuan, Q.,Guan, Y.,Xia, N.
Lineage-mosaic and mutation-patched spike proteins for broad-spectrum COVID-19 vaccine.
Cell Host Microbe, 30:1732-1744.e7, 2022
Cited by
PubMed Abstract: SARS-CoV-2 spread in humans results in continuous emergence of new variants, highlighting the need for vaccines with broad-spectrum antigenic coverage. Using inter-lineage chimera and mutation-patch strategies, we engineered a recombinant monomeric spike variant (STFK1628x) that contains key regions and residues across multiple SAR-CoV-2 variants. STFK1628x demonstrated high immunogenicity and mutually complementary antigenicity to its prototypic form (STFK). In hamsters, a bivalent vaccine composed of STFK and STFK1628x elicited high titers of broad-spectrum neutralizing antibodies to 19 circulating SARS-CoV-2 variants, including Omicron sublineages BA.1, BA.1.1, BA.2, BA.2.12.1, BA.2.75, and BA.4/5. Furthermore, this vaccine conferred robust protection against intranasal challenges by either SARS-CoV-2 ancestral strain or immune-evasive Beta and Omicron BA.1. Strikingly, vaccination with the bivalent vaccine in hamsters effectively blocked within-cage virus transmission of ancestral SARS-CoV-2, Beta variant, and Omicron BA.1 to unvaccinated sentinels. Thus, our study provided insight and antigen candidates for the development of next-generation COVID-19 vaccines.
PubMed: 36323313
DOI: 10.1016/j.chom.2022.10.011
主引用文献が同じPDBエントリー
実験手法
ELECTRON MICROSCOPY (3.81 Å)
構造検証レポート
Validation report summary of 7wp6
検証レポート(詳細版)ダウンロードをダウンロード

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件を2025-07-23に公開中

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