7WOI

Structure of the shaft pilin Spa2 from Corynebacterium glutamicum

Summary for 7WOI

• Entry DOI: 10.2210/pdb7woi/pdb
• Descriptor: Spa2, 1,2-ETHANEDIOL, GLYCEROL, ... (4 entities in total)
• Functional Keywords: bacterial pilus, adhesion, self-assembly, sortase catelyzed pilus, cell adhesion
• Biological source: Corynebacterium glutamicum ATCC 14067
• Total number of polymer chains: 2
• Total formula weight: 106448.57

Authors

Wu, Y.F.,Wang, L.T.,Huang, Y.Y.,Zhong, C.,Zhou, J. (deposition date: 2022-01-21, release date: 2023-01-25, Last modification date: 2024-11-06)

Primary citation

Huang, Y.,Wu, Y.,Hu, H.,Tong, B.,Wang, J.,Zhang, S.,Wang, Y.,Zhang, J.,Yin, Y.,Dai, S.,Zhao, W.,An, B.,Pu, J.,Wang, Y.,Peng, C.,Li, N.,Zhou, J.,Tan, Y.,Zhong, C.
Accelerating the design of pili-enabled living materials using an integrative technological workflow.
Nat.Chem.Biol., 2023

PubMed Abstract: Bacteria can be programmed to create engineered living materials (ELMs) with self-healing and evolvable functionalities. However, further development of ELMs is greatly hampered by the lack of engineerable nonpathogenic chassis and corresponding programmable endogenous biopolymers. Here, we describe a technological workflow for facilitating ELMs design by rationally integrating bioinformatics, structural biology and synthetic biology technologies. We first develop bioinformatics software, termed Bacteria Biopolymer Sniffer (BBSniffer), that allows fast mining of biopolymers and biopolymer-producing bacteria of interest. As a proof-of-principle study, using existing pathogenic pilus as input, we identify the covalently linked pili (CLP) biosynthetic gene cluster in the industrial workhorse Corynebacterium glutamicum. Genetic manipulation and structural characterization reveal the molecular mechanism of the CLP assembly, ultimately enabling a type of programmable pili for ELM design. Finally, engineering of the CLP-enabled living materials transforms cellulosic biomass into lycopene by coupling the extracellular and intracellular bioconversion ability.

PubMed: 38012344
DOI: 10.1038/s41589-023-01489-x

Experimental method

X-RAY DIFFRACTION (2.73 Å)