7WLY

Cryo-EM structure of the Omicron S in complex with 35B5 Fab(1 down- and 2 up RBDs)

7WLY の概要

• エントリーDOI: 10.2210/pdb7wly/pdb
• EMDBエントリー: 32594
• 分子名称: Spike glycoprotein, Heavy chain of 35B5 Fab, Light chain of 35B5 Fab, ... (5 entities in total)
• 機能のキーワード: viral protein, immune system
• 由来する生物種: Severe acute respiratory syndrome coronavirus 2; 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 532669.78

構造登録者

Wang, X.,Zhu, Y. (登録日: 2022-01-14, 公開日: 2022-05-25, 最終更新日: 2024-11-13)

主引用文献

Wang, X.,Chen, X.,Tan, J.,Yue, S.,Zhou, R.,Xu, Y.,Lin, Y.,Yang, Y.,Zhou, Y.,Deng, K.,Chen, Z.,Ye, L.,Zhu, Y.
35B5 antibody potently neutralizes SARS-CoV-2 Omicron by disrupting the N-glycan switch via a conserved spike epitope.
Cell Host Microbe, 30:887-, 2022

PubMed Abstract: The SARS-CoV-2 Omicron variant harbors more than 30 mutations in the spike protein, leading to immune evasion from many therapeutic neutralizing antibodies. We reveal that a receptor-binding domain (RBD)-targeting monoclonal antibody, 35B5, exhibits potent neutralizing efficacy to Omicron. Cryo-electron microscopy structures of the extracellular domain trimer of Omicron spike with 35B5 Fab reveal that Omicron spike exhibits tight trimeric packing and high thermostability, as well as significant antigenic shifts and structural changes, within the RBD, N-terminal domain (NTD), and subdomains 1 and 2. However, these changes do not affect targeting of the invariant 35B5 epitope. 35B5 potently neutralizes SARS-CoV-2 Omicron and other variants by causing significant conformational changes within a conserved N-glycan switch that controls the transition of RBD from the "down" state to the "up" state, which allows recognition of the host entry receptor ACE2. This mode of action and potent neutralizing capacity of 35B5 indicate its potential therapeutic application for SARS-CoV-2.

PubMed: 35436443
DOI: 10.1016/j.chom.2022.03.035

実験手法

ELECTRON MICROSCOPY (3.4 Å)