7WKC
A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability
Summary for 7WKC
| Entry DOI | 10.2210/pdb7wkc/pdb |
| Descriptor | Carboxysome shell vertex protein CcmL (1 entity in total) |
| Functional Keywords | protein cages, quantum dots, cryoelectron microscopy, biosynthetic protein |
| Biological source | Thermosynechococcus vestitus BP-1 |
| Total number of polymer chains | 5 |
| Total formula weight | 57709.62 |
| Authors | |
| Primary citation | Gao, R.,Tan, H.,Li, S.,Ma, S.,Tang, Y.,Zhang, K.,Zhang, Z.,Fan, Q.,Yang, J.,Zhang, X.E.,Li, F. A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability. Proc.Natl.Acad.Sci.USA, 119:-, 2022 Cited by PubMed Abstract: Protein nanocages (PNCs) in cells and viruses have inspired the development of self-assembling protein nanomaterials for various purposes. Despite the successful creation of artificial PNCs, the de novo design of PNCs with defined permeability remains challenging. Here, we report a prototype oxygen-impermeable PNC (OIPNC) assembled from the vertex protein of the β-carboxysome shell, CcmL, with quantum dots as the template via interfacial engineering. The structure of the cage was solved at the atomic scale by combined solid-state NMR spectroscopy and cryoelectron microscopy, showing icosahedral assembly of CcmL pentamers with highly conserved interpentamer interfaces. Moreover, a gating mechanism was established by reversibly blocking the pores of the cage with molecular patches. Thus, the oxygen permeability, which was probed by an oxygen sensor inside the cage, can be completely controlled. The CcmL OIPNC represents a PNC platform for oxygen-sensitive or oxygen-responsive storage, catalysis, delivery, sensing, etc. PubMed: 35078933DOI: 10.1073/pnas.2104964119 PDB entries with the same primary citation |
| Experimental method | SOLID-STATE NMR |
Structure validation
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