7WKC

A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability

7WKC の概要

• エントリーDOI: 10.2210/pdb7wkc/pdb
• 分子名称: Carboxysome shell vertex protein CcmL (1 entity in total)
• 機能のキーワード: protein cages, quantum dots, cryoelectron microscopy, biosynthetic protein
• 由来する生物種: Thermosynechococcus vestitus BP-1
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 57709.62

構造登録者

Tan, H.,Yang, J. (登録日: 2022-01-08, 公開日: 2022-02-16, 最終更新日: 2024-05-15)

主引用文献

Gao, R.,Tan, H.,Li, S.,Ma, S.,Tang, Y.,Zhang, K.,Zhang, Z.,Fan, Q.,Yang, J.,Zhang, X.E.,Li, F.
A prototype protein nanocage minimized from carboxysomes with gated oxygen permeability.
Proc.Natl.Acad.Sci.USA, 119:-, 2022

PubMed Abstract: Protein nanocages (PNCs) in cells and viruses have inspired the development of self-assembling protein nanomaterials for various purposes. Despite the successful creation of artificial PNCs, the de novo design of PNCs with defined permeability remains challenging. Here, we report a prototype oxygen-impermeable PNC (OIPNC) assembled from the vertex protein of the β-carboxysome shell, CcmL, with quantum dots as the template via interfacial engineering. The structure of the cage was solved at the atomic scale by combined solid-state NMR spectroscopy and cryoelectron microscopy, showing icosahedral assembly of CcmL pentamers with highly conserved interpentamer interfaces. Moreover, a gating mechanism was established by reversibly blocking the pores of the cage with molecular patches. Thus, the oxygen permeability, which was probed by an oxygen sensor inside the cage, can be completely controlled. The CcmL OIPNC represents a PNC platform for oxygen-sensitive or oxygen-responsive storage, catalysis, delivery, sensing, etc.

PubMed: 35078933
DOI: 10.1073/pnas.2104964119

実験手法

SOLID-STATE NMR