7WJ0

Solution structure of N-terminal domain (nMazE6) of mycobacterial antitoxin MazE6 from MazEF6 TA system

7WJ0 の概要

• エントリーDOI: 10.2210/pdb7wj0/pdb
• 分子名称: Antitoxin MazE6 (1 entity in total)
• 機能のキーワード: rhh domain, mycobacterial ta system, mazef6, dna binding domain, antitoxin
• 由来する生物種: Mycobacterium tuberculosis H37Rv
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 11685.07

構造登録者

Kumari, K.,Sarma, S.P. (登録日: 2022-01-05, 公開日: 2022-10-12, 最終更新日: 2024-05-15)

主引用文献

Kumari, K.,Sarma, S.P.
Structural and mutational analysis of MazE6-operator DNA complex provide insights into autoregulation of toxin-antitoxin systems.
Commun Biol, 5:963-963, 2022

PubMed Abstract: Of the 10 paralogs of MazEF Toxin-Antitoxin system in Mycobacterium tuberculosis, MazEF6 plays an important role in multidrug tolerance, virulence, stress adaptation and Non Replicative Persistant (NRP) state establishment. The solution structures of the DNA binding domain of MazE6 and of its complex with the cognate operator DNA show that transcriptional regulation occurs by binding of MazE6 to an 18 bp operator sequence bearing the TANNNT motif (-10 region). Kinetics and thermodynamics of association, as determined by NMR and ITC, indicate that the nMazE6-DNA complex is of high affinity. Residues in N-terminal region of MazE6 that are key for its homodimerization, DNA binding specificity, and the base pairs in the operator DNA essential for the protein-DNA interaction, have been identified. It provides a basis for design of chemotherapeutic agents that will act via disruption of TA autoregulation, leading to cell death.

PubMed: 36109664
DOI: 10.1038/s42003-022-03933-5

実験手法

SOLUTION NMR