7WGH

Crystal structure of AflSQS from Aspergillus flavus in complex with FSPP

7WGH の概要

• エントリーDOI: 10.2210/pdb7wgh/pdb
• 分子名称: Squalene synthase, PYROPHOSPHATE 2-, PHOSPHATE ION, ... (5 entities in total)
• 機能のキーワード: isoprenoid synthase, biosynthetic protein
• 由来する生物種: Aspergillus flavus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55994.65

構造登録者

Shang, N.,Liu, W.D.,Chen, C.C.,Guo, R.T. (登録日: 2021-12-28, 公開日: 2022-11-09, 最終更新日: 2023-11-29)

主引用文献

Malwal, S.R.,Shang, N.,Liu, W.,Li, X.,Zhang, L.,Chen, C.C.,Guo, R.T.,Oldfield, E.
A Structural and Bioinformatics Investigation of a Fungal Squalene Synthase and Comparisons with Other Membrane Proteins.
Acs Omega, 7:22601-22612, 2022

PubMed Abstract: There is interest in the development of drugs to treat fungal infections due to the increasing threat of drug resistance, and here, we report the first crystallographic structure of the catalytic domain of a fungal squalene synthase (SQS), SQS (AfSQS), a potential drug target, together with a bioinformatics study of fungal, human, and protozoal SQSs. Our X-ray results show strong structural similarities between the catalytic domains in these proteins, but, remarkably, using bioinformatics, we find that there is also a large, highly polar helix in the fungal proteins that connects the catalytic and membrane-anchoring transmembrane domains. This polar helix is absent in squalene synthases from all other lifeforms. We show that the transmembrane domain in AfSQS and in other SQSs, stannin, and steryl sulfatase, have very similar properties (% polar residues, hydrophobicity, and hydrophobic moment) to those found in the "penultimate" C-terminal helical domain in squalene epoxidase, while the final C-terminal domain in squalene epoxidase is more polar and may be monotopic. We also propose structural models for full-length AfSQS based on the bioinformatics results as well as a deep learning program that indicate that the C-terminus region may also be membrane surface-associated. Taken together, our results are of general interest given the unique nature of the polar helical domain in fungi that may be involved in protein-protein interactions as well as being a future target for antifungals.

PubMed: 35811857
DOI: 10.1021/acsomega.2c01924

実験手法

X-RAY DIFFRACTION (2.36 Å)