7WFF

Subcomplexes B,M and L in the Cylic electron transfer supercomplex NDH-PSI from Arabidopsis

7WFF の概要

• エントリーDOI: 10.2210/pdb7wff/pdb
• EMDBエントリー: 32464
• 分子名称: NAD(P)H-quinone oxidoreductase subunit 1, chloroplastic, Photosynthetic NDH subunit of subcomplex B 3, chloroplastic, NDH dependent flow 6, ... (20 entities in total)
• 機能のキーワード: subcomplex b, subcomplex m, subcomplex l, arabidopisis, plant, cyclic electron transport supercomplex, electron transport
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 17
• 化学式量合計: 565367.80

構造登録者

Pan, X.W.,Li, M. (登録日: 2021-12-26, 公開日: 2022-03-16, 最終更新日: 2025-06-25)

主引用文献

Su, X.,Cao, D.,Pan, X.,Shi, L.,Liu, Z.,Dall'Osto, L.,Bassi, R.,Zhang, X.,Li, M.
Supramolecular assembly of chloroplast NADH dehydrogenase-like complex with photosystem I from Arabidopsis thaliana.
Mol Plant, 15:454-467, 2022

PubMed Abstract: Cyclic electron transport/flow (CET/CEF) in chloroplasts is a regulatory process essential for the optimization of plant photosynthetic efficiency. A crucial CEF pathway is catalyzed by a membrane-embedded NADH dehydrogenase-like (NDH) complex that contains at least 29 protein subunits and associates with photosystem I (PSI) to form the NDH-PSI supercomplex. Here, we report the 3.9 Å resolution structure of the Arabidopsis thaliana NDH-PSI (AtNDH-PSI) supercomplex. We constructed structural models for 26 AtNDH subunits, among which 11 are unique to chloroplasts and stabilize the core part of the NDH complex. In the supercomplex, one NDH can bind up to two PSI-light-harvesting complex I (PSI-LHCI) complexes at both sides of its membrane arm. Two minor LHCIs, Lhca5 and Lhca6, each present in one PSI-LHCI, interact with NDH and contribute to supercomplex formation and stabilization. Collectively, our study reveals the structural details of the AtNDH-PSI supercomplex assembly and provides a molecular basis for further investigation of the regulatory mechanism of CEF in plants.

PubMed: 35123031
DOI: 10.1016/j.molp.2022.01.020

実験手法

ELECTRON MICROSCOPY (3.59 Å)