7WBO

Crystal structure of Sarcoplasmic Calcium-Binding Protein from Scylla paramamosain

7WBO の概要

• エントリーDOI: 10.2210/pdb7wbo/pdb
• 分子名称: Sarcoplasmic calcium-binding protein, 1,2-ETHANEDIOL, CALCIUM ION, ... (5 entities in total)
• 機能のキーワード: allergy, scylla paramamosain, ef-hand, allergen
• 由来する生物種: Scylla paramamosain (green mud crab)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22977.83

構造登録者

Chen, Y.,Jin, T.,Liu, G. (登録日: 2021-12-17, 公開日: 2022-12-21, 最終更新日: 2023-11-29)

主引用文献

Chen, Y.,Jin, T.,Li, M.,Yun, X.,Huan, F.,Liu, Q.,Hu, M.,Wei, X.,Zheng, P.,Liu, G.
Crystal Structure Analysis of Sarcoplasmic-Calcium-Binding Protein: An Allergen in Scylla paramamosain.
J.Agric.Food Chem., 71:1214-1223, 2023

PubMed Abstract: The structure of allergenic proteins provides important information about the binding of allergens to antibodies. In this study, the crystal structure of Scy p 4 with a resolution of 1.60 Å was obtained by X-ray diffraction. Epitope mapping of Scy p 4 revealed that linear epitopes are located on the surface of Scy p 4. Also, conformational epitopes are mostly located in the structural conservative region. Further structural comparison, surface electrostatic potential, and hydrogen bond force analysis showed that mutation of Asp and Asp would lead to calcium-binding capacity being lost and destruction of allergenicity. Furthermore, a comparative analysis of structure showed that sarcoplasmic-calcium-binding protein (SCP) had high sequence, secondary, and spatial structural identity in crustaceans, which may be an important factor leading to cross-reactivity among crustaceans. The structure of Scy p 4 provides a template for epitope evaluation and localization of SCPs, which will help to reveal cross-reactivity among species.

PubMed: 36602420
DOI: 10.1021/acs.jafc.2c07267

実験手法

X-RAY DIFFRACTION (1.6 Å)