7WBK

Crystal structure of Legionella pneumophila effector protein Lpg0081

7WBK の概要

• エントリーDOI: 10.2210/pdb7wbk/pdb
• 分子名称: Lpg0081, SULFATE ION (3 entities in total)
• 機能のキーワード: macrod-type adp-ribose hydrolase, hydrolase
• 由来する生物種: Legionella pneumophila subsp. pneumophila str. Philadelphia 1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 94693.63

構造登録者

Lee, J.,Kim, H.,Oh, B.H. (登録日: 2021-12-16, 公開日: 2022-06-15, 最終更新日: 2024-10-16)

主引用文献

Kubori, T.,Lee, J.,Kim, H.,Yamazaki, K.,Nishikawa, M.,Kitao, T.,Oh, B.H.,Nagai, H.
Reversible modification of mitochondrial ADP/ATP translocases by paired Legionella effector proteins.
Proc.Natl.Acad.Sci.USA, 119:e2122872119-e2122872119, 2022

PubMed Abstract: Adenosine diphosphate (ADP) ribosylation is a reversible posttranslational modification involved in the regulation of numerous cellular processes. Prototype ADP ribosyltransferases (ARTs) from many pathogenic bacteria are known to function as toxins, while other bacterial ARTs have just recently emerged. Recent studies have shown that bacteria also possess enzymes that function as poly-ADP ribose (ADPr) glycohydrolases (PARGs), which reverse poly-ADP ribosylation. However, how bacteria manipulate host target proteins by coordinated reactions of ARTs and ADPr hydrolases (ARHs) remains elusive. The intracellular bacterial pathogen Legionella pneumophila, the causative agent of Legionnaires’ disease, transports a large array of effector proteins via the Dot/Icm type IV secretion system to host cells. The effector proteins, which mostly function as enzymes, modulate host cellular processes for the bacteria’s benefit. In this study, we identified a pair of L. pneumophila effector proteins, Lpg0080 and Lpg0081, which function as an ART and an ARH, respectively. The two proteins were shown to coordinately modulate mitochondrial ADP/adenosine triphosphate (ATP) translocases (ANTs) by their enzymatic activities to conjugate ADPr to, and remove it from, a key arginine residue. The crystal structures of Lpg0081 and the Lpg0081:ADPr complex indicated that Lpg0081 is a macroD-type ARH with a noncanonical macrodomain, whose folding topology is strikingly distinct from that of the canonical macrodomain that is ubiquitously found in eukaryotic PARGs and ARHs. Our results illustrate that L. pneumophila has acquired an effector pair that coordinately manipulate mitochondrial activity via reversible chemical modification of ANTs.

PubMed: 35653564
DOI: 10.1073/pnas.2122872119

実験手法

X-RAY DIFFRACTION (2.74 Å)