7WB3

Crystal structure of T. maritima Rex in ternary complex

7WB3 の概要

• エントリーDOI: 10.2210/pdb7wb3/pdb
• 分子名称: Redox-sensing transcriptional repressor Rex, DNA (5'-D(*AP*TP*TP*TP*GP*AP*GP*AP*AP*AP*TP*TP*TP*AP*TP*CP*AP*CP*AP*AP*AP*A)-3'), DNA (5'-D(*TP*TP*TP*TP*GP*TP*GP*AP*TP*AP*AP*AP*TP*TP*TP*CP*TP*CP*AP*AP*AP*T)-3'), ... (5 entities in total)
• 機能のキーワード: rex, nicotinamide adenine dinucleotide, transcriptional regulator, ternary complex, dna binding protein, gene regulation-dna complex, gene regulation/dna
• 由来する生物種: Thermotoga maritima MSB8; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 60796.96

構造登録者

Lee, J.Y.,Jeong, K.H.,Lee, H.J.,Park, Y.W. (登録日: 2021-12-15, 公開日: 2022-02-16, 最終更新日: 2023-11-29)

主引用文献

Jeong, K.H.,Lee, H.J.,Park, Y.W.,Lee, J.Y.
Structural Basis of Redox-Sensing Transcriptional Repressor Rex with Cofactor NAD + and Operator DNA.
Int J Mol Sci, 23:-, 2022

PubMed Abstract: The transcriptional repressor Rex plays important roles in regulating the expression of respiratory genes by sensing the reduction-oxidation (redox) state according to the intracellular NAD/NADH balance. Previously, we reported on crystal structures of apo, NAD-bound, and NADH-bound forms of Rex from to analyze the structural basis of transcriptional regulation depending on either NAD or NADH binding. In this study, the crystal structure of Rex in ternary complex with NAD and operator DNA revealed that the -terminal domain of Rex, including the helix-turn-helix motif, forms extensive contacts with DNA in addition to DNA sequence specificity. Structural comparison of the Rex in apo, NAD-bound, NADH-bound, and ternary complex forms provides a comprehensive picture of transcriptional regulation in the Rex. These data demonstrate that the conformational change in Rex when binding with the reduced NADH or oxidized NAD determines operator DNA binding. The movement of the -terminal domains toward the operator DNA was blocked upon binding of NADH ligand molecules. The structural results provide insights into the molecular mechanism of Rex binding with operator DNA and cofactor NAD/NADH, which is conserved among Rex family repressors. Structural analysis of Rex from also supports the previous hypothesis about the NAD/NADH-specific transcriptional regulation mechanism of Rex homologues.

PubMed: 35163512
DOI: 10.3390/ijms23031578

実験手法

X-RAY DIFFRACTION (2.401 Å)