7WA4

Crystal structure of GIGANTEA in complex with LKP2

7WA4 の概要

• エントリーDOI: 10.2210/pdb7wa4/pdb
• 分子名称: Protein GIGANTEA, Adagio protein 2, FLAVIN MONONUCLEOTIDE (3 entities in total)
• 機能のキーワード: gi, lov domain, flavin-mononucleotide, circadian clock protein
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 105216.04

構造登録者

Pathak, D.,Dahal, P.,Kwon, E.,Kim, D.Y. (登録日: 2021-12-12, 公開日: 2022-04-27, 最終更新日: 2024-10-16)

主引用文献

Kwon, E.,Pathak, D.,Dahal, P.,Tandukar, S.,Jung, H.S.,Kim, W.Y.,Kim, D.Y.
Structural analysis of the regulation of blue-light receptors by GIGANTEA.
Cell Rep, 39:110700-110700, 2022

PubMed Abstract: In Arabidopsis, GIGANTEA (GI), together with the blue-light receptors ZTL, LKP2, and FKF1, regulates degradation of the core clock protein TOC1 and the flowering repressor CDFs, thereby controlling circadian oscillation and flowering. Despite the significance of GI in diverse plant physiology, its molecular function is not much understood because of technical problems in protein preparation and a lack of structural information. Here, we report the purification of the GI monomer and the crystal structure of the GI/LKP2 complex. The crystal structure reveals that residues 1-813 of GI possess an elongated rigid structure formed by stacking hydrophobic α-helices and that the LOV domain of LKP2 binds to the middle region of the GI (residues 563-789). Interaction analysis further shows that LOV homodimers are converted to monomers by GI binding. Our results provide structural insights into the regulation of the circadian clock and photoperiodic flowering by GI and ZTL/LKP2/FKF1.

PubMed: 35443175
DOI: 10.1016/j.celrep.2022.110700

実験手法

X-RAY DIFFRACTION (3.5 Å)