7W7V

'late' E2P of SERCA2b

7W7V の概要

• エントリーDOI: 10.2210/pdb7w7v/pdb
• EMDBエントリー: 32349
• 分子名称: Sarcoplasmic/endoplasmic reticulum calcium ATPase 2, BERYLLIUM TRIFLUORIDE ION, MAGNESIUM ION (3 entities in total)
• 機能のキーワード: calcium, metal transport
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 114959.98

構造登録者

Zhang, Y.,Watanabe, S.,Tsutsumi, A.,Inaba, K. (登録日: 2021-12-06, 公開日: 2022-12-14, 最終更新日: 2025-06-18)

主引用文献

Zhang, Y.,Kobayashi, C.,Cai, X.,Watanabe, S.,Tsutsumi, A.,Kikkawa, M.,Sugita, Y.,Inaba, K.
Multiple sub-state structures of SERCA2b reveal conformational overlap at transition steps during the catalytic cycle.
Cell Rep, 41:111760-111760, 2022

PubMed Abstract: Sarco/endoplasmic reticulum Ca ATPase (SERCA) pumps Ca into the endoplasmic reticulum (ER). Herein, we present cryo-electron microscopy (EM) structures of three intermediates of SERCA2b: Ca-bound phosphorylated (E1P·2Ca) and Ca-unbound dephosphorylated (E2·Pi) intermediates and another between the E2P and E2·Pi states. Our cryo-EM analysis demonstrates that the E1P·2Ca state exists in low abundance and preferentially transitions to an E2P-like structure by releasing Ca and that the Ca release gate subsequently undergoes stepwise closure during the dephosphorylation processes. Importantly, each intermediate adopts multiple sub-state structures including those like the next one in the catalytic series, indicating conformational overlap at transition steps, as further substantiated by atomistic molecular dynamic simulations of SERCA2b in a lipid bilayer. The present findings provide insight into how enzymes accelerate catalytic cycles.

PubMed: 36476867
DOI: 10.1016/j.celrep.2022.111760

実験手法

ELECTRON MICROSCOPY (3 Å)