7W4P

The structure of KATP H175K mutant in closed state

7W4P の概要

• エントリーDOI: 10.2210/pdb7w4p/pdb
• EMDBエントリー: 32311
• 分子名称: ATP-sensitive inward rectifier potassium channel 11, ATP-binding cassette sub-family C member 8 isoform X2, ADENOSINE-5'-DIPHOSPHATE, ... (6 entities in total)
• 機能のキーワード: katp, kir6.2, sur1, nn414, adp, atp, transport protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 890417.03

構造登録者

Chen, L.,Wang, M. (登録日: 2021-11-28, 公開日: 2022-06-01, 最終更新日: 2024-10-30)

主引用文献

Wang, M.,Wu, J.X.,Ding, D.,Chen, L.
Structural insights into the mechanism of pancreatic K ATP channel regulation by nucleotides.
Nat Commun, 13:2770-2770, 2022

PubMed Abstract: ATP-sensitive potassium channels (K) are metabolic sensors that convert the intracellular ATP/ADP ratio to the excitability of cells. They are involved in many physiological processes and implicated in several human diseases. Here we present the cryo-EM structures of the pancreatic K channel in both the closed state and the pre-open state, resolved in the same sample. We observe the binding of nucleotides at the inhibitory sites of the Kir6.2 channel in the closed but not in the pre-open state. Structural comparisons reveal the mechanism for ATP inhibition and Mg-ADP activation, two fundamental properties of K channels. Moreover, the structures also uncover the activation mechanism of diazoxide-type K openers.

PubMed: 35589716
DOI: 10.1038/s41467-022-30430-4

実験手法

ELECTRON MICROSCOPY (3.19 Å)