7W2I

Crystal structure of LOG (Rv1205) from Mycobacterium tuberculosis

7W2I の概要

• エントリーDOI: 10.2210/pdb7w2i/pdb
• 分子名称: Cytokinin riboside 5'-monophosphate phosphoribohydrolase, MAGNESIUM ION, 1,2-ETHANEDIOL, ... (7 entities in total)
• 機能のキーワード: log, rv1205, mycobacterium tuberculosis, hydrolase
• 由来する生物種: Mycobacterium tuberculosis; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 77152.68

構造登録者

Shang, L.,Zhang, G. (登録日: 2021-11-23, 公開日: 2022-07-06, 最終更新日: 2023-11-29)

主引用文献

Shang, L.,Li, G.,Lin, Q.,Ou, M.,Liang, J.,Xiao, G.,Wang, Z.,Cui, S.,Zhang, T.,Liu, L.,Zhang, G.
Crystal structure of the cytokinin-producing enzyme "lonely guy" (LOG) from Mycobacterium tuberculosis.
Biochem.Biophys.Res.Commun., 598:113-118, 2022

PubMed Abstract: Mycobacterium tuberculosis (Mtb) is an extremely successful intracellular pathogen that cause a large number of death worldwide. It is interesting that this non-phytopathogen can synthesize cytokinin by "lonely guy" (LOG) protein. The cytokinin biosynthesis pathway in Mtb is not clear. Here we determined the crystal structure of LOG from Mtb (MtLOG) at a high resolution of 1.8 Å. MtLOG exists as dimer which belongs to type-I LOG and shows a typical α-β Rossmann fold. Like other LOGs, MtLOG also contains a conserved "PGGXGTXXE" motif that contributes to the formation of an active site. For the first time, we found that the MtLOG binds to Mg in the negative potential pocket. According to the docking result, we found that Arg78, Arg98 and Tyr162 should be the key amino acid involved in substrate binding. Our findings provide a structural basis for cytokinin study in Mtb and will play an important role in design and development of enzyme inhibitors.

PubMed: 35158209
DOI: 10.1016/j.bbrc.2022.01.103

実験手法

X-RAY DIFFRACTION (1.8 Å)