7W1C

Crystal structure of Klebsiella pneumoniae K1 capsule-specific polysaccharide lyase in a P1 crystal form

This is a non-PDB format compatible entry.

Summary for 7W1C

• Entry DOI: 10.2210/pdb7w1c/pdb
• Descriptor: K1 LYASE, (2S)-2-hydroxybutanedioic acid, IMIDAZOLE, ... (5 entities in total)
• Functional Keywords: beta-helix, polysaccharide lyase, tail spike protein, viral protein
• Biological source: Klebsiella phage NTUH-K2044-K1-1
• Total number of polymer chains: 6
• Total formula weight: 436969.05

Authors

Tu, I.F.,Huang, K.F.,Wu, S.H. (deposition date: 2021-11-19, release date: 2022-05-18, Last modification date: 2024-05-29)

Primary citation

Tu, I.F.,Lin, T.L.,Yang, F.L.,Lee, I.M.,Tu, W.L.,Liao, J.H.,Ko, T.P.,Wu, W.J.,Jan, J.T.,Ho, M.R.,Chou, C.Y.,Wang, A.H.,Wu, C.Y.,Wang, J.T.,Huang, K.F.,Wu, S.H.
Structural and biological insights into Klebsiella pneumoniae surface polysaccharide degradation by a bacteriophage K1 lyase: implications for clinical use.
J.Biomed.Sci., 29:9-9, 2022

PubMed Abstract: K1 capsular polysaccharide (CPS)-associated Klebsiella pneumoniae is the primary cause of pyogenic liver abscesses (PLA) in Asia. Patients with PLA often have serious complications, ultimately leading to a mortality of ~ 5%. This K1 CPS has been reported as a promising target for development of glycoconjugate vaccines against K. pneumoniae infection. The pyruvylation and O-acetylation modifications on the K1 CPS are essential to the immune response induced by the CPS. To date, however, obtaining the fragments of K1 CPS that contain the pyruvylation and O-acetylation for generating glycoconjugate vaccines still remains a challenge.

PubMed: 35130876
DOI: 10.1186/s12929-022-00792-4

Experimental method

X-RAY DIFFRACTION (1.48 Å)