7W14

Coxsackievirus B3 at pH7.4 (VP3-234E) incubation with coxsackievirus and adenovirus receptor for 20min

Summary for 7W14

• Entry DOI: 10.2210/pdb7w14/pdb
• EMDB information: 32250
• Descriptor: Capsid protein VP1, Capsid protein VP2, Capsid protein VP3, ... (5 entities in total)
• Functional Keywords: cvb3, vp3-234e, coxsackievirus and adenovirus receptor, 20min, virus
• Biological source: Coxsackievirus B3; More
• Total number of polymer chains: 5
• Total formula weight: 118156.01

Authors

Wang, Q.L.,Liu, C.C. (deposition date: 2021-11-19, release date: 2022-01-19, Last modification date: 2022-08-03)

Primary citation

Wang, Q.,Yang, Q.,Liu, C.,Wang, G.,Song, H.,Shang, G.,Peng, R.,Qu, X.,Liu, S.,Cui, Y.,Wang, P.,Xu, W.,Zhao, X.,Qi, J.,Yang, M.,Gao, G.F.
Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains.
Proc.Natl.Acad.Sci.USA, 119:-, 2022

PubMed Abstract: Receptor usage defines cell tropism and contributes to cell entry and infection. Coxsackievirus B (CVB) engages coxsackievirus and adenovirus receptor (CAR), and selectively utilizes the decay-accelerating factor (DAF; CD55) to infect cells. However, the differential receptor usage mechanism for CVB remains elusive. This study identified VP3-234 residues (234Q/N/V/D/E) as critical population selection determinants during CVB3 virus evolution, contributing to diverse binding affinities to CD55. Cryoelectron microscopy (cryo-EM) structures of CD55-binding/nonbinding isolates and their complexes with CD55 or CAR were obtained under both neutral and acidic conditions, and the molecular mechanism of VP3-234 residues determining CD55 affinity/specificity for naturally occurring CVB3 strains was elucidated. Structural and biochemical studies in vitro revealed the dynamic entry process of CVB3 and the function of the uncoating receptor CAR with different pH preferences. This work provides detailed insight into the molecular mechanism of CVB infection and contributes to an in-depth understanding of enterovirus attachment receptor usage.

PubMed: 35046043
DOI: 10.1073/pnas.2118590119

Experimental method

ELECTRON MICROSCOPY (2.2 Å)