7W12

Complex structure of alginate lyase AlyB-OU02 with G9

7W12 の概要

• エントリーDOI: 10.2210/pdb7w12/pdb
• 分子名称: Alginate lyase, alpha-L-gulopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid-(1-4)-alpha-L-gulopyranuronic acid, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: pl7 family, alginate lyase, complex, lyase
• 由来する生物種: Vibrio splendidus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55817.70

構造登録者

Liu, W.Z.,Lyu, Q.Q.,Zhang, K.K. (登録日: 2021-11-19, 公開日: 2022-11-16, 最終更新日: 2024-10-09)

主引用文献

Zhang, K.,Li, Z.,Zhu, Q.,Cao, H.,He, X.,Zhang, X.H.,Liu, W.,Lyu, Q.
Determination of oligosaccharide product distributions of PL7 alginate lyases by their structural elements.
Commun Biol, 5:782-782, 2022

PubMed Abstract: Alginate lyases can be used to produce well-defined alginate oligosaccharides (AOSs) because of their specificities for AOS products. A large number of alginate lyases have been recorded in the CAZy database; however, the majority are annotated-only alginate lyases that include little information on their products, thus limiting their applications. Here, we establish a simple and experiment-saving approach to predict product distributions for PL7 alginate lyases through extensive structural biology, bioinformatics and biochemical studies. Structural study on several PL7 alginate lyases reveals that two loops around the substrate binding cleft determine product distribution. Furthermore, a database containing the loop information of all annotated-only single-domain PL7 alginate lyases is constructed, enabling systematic exploration of the association between loop and product distribution. Based on these results, a simplified loop/product distribution relationship is proposed, giving us information on product distribution directly from the amino acid sequence.

PubMed: 35918517
DOI: 10.1038/s42003-022-03721-1

実験手法

X-RAY DIFFRACTION (2.25 Å)