7W07

Itaconate inducible LysR-Type Transcriptional regulator (ITCR) in complex with itaconate, Space group C121.

7W07 の概要

• エントリーDOI: 10.2210/pdb7w07/pdb
• 分子名称: Transcriptional regulator, LysR family, 2-methylidenebutanedioic acid, SULFATE ION, ... (4 entities in total)
• 機能のキーワード: lttr, itcr, itaconate, transcription
• 由来する生物種: Yersinia pseudotuberculosis serotype O:3 (strain YPIII)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 32484.21

構造登録者

Sun, P.K.,Wang, B.,Li, X.J. (登録日: 2021-11-17, 公開日: 2022-10-26, 最終更新日: 2023-11-29)

主引用文献

Sun, P.,Zhang, Z.,Wang, B.,Liu, C.,Chen, C.,Liu, P.,Li, X.
A genetically encoded fluorescent biosensor for detecting itaconate with subcellular resolution in living macrophages.
Nat Commun, 13:6562-6562, 2022

PubMed Abstract: Itaconate is a newly discovered endogenous metabolite promoting an anti-inflammatory program during innate immune response, but the precise mechanisms underlying its effect remains poorly understood owing primarily to the limitations of available itaconate-monitoring techniques. Here, we develop and validate a genetically encoded fluorescent itaconate biosensor, BioITA, for directly monitoring itaconate dynamics in subcellular compartments of living macrophages. Utilizing BioITA, we monitor the itaconate dynamics in response to lipopolysaccharide (LPS) stimulation in the context of modulating itaconate transportation and metabolism. Moreover, we show that STING activation induces itaconate production, and injection of AAVs expressing cytosolic BioITA into mice allows directly reporting elevation of itaconate level in activated macrophages derived from LPS-injected mice. Thus, BioITA enables subcellular resolution imaging of itaconate in living macrophages.

PubMed: 36333306
DOI: 10.1038/s41467-022-34306-5

実験手法

X-RAY DIFFRACTION (1.48 Å)