7W01

Cryo-EM structure of nucleotide-free ABCA3

7W01 の概要

• エントリーDOI: 10.2210/pdb7w01/pdb
• EMDBエントリー: 32233
• 分子名称: Phospholipid-transporting ATPase ABCA3, 2-acetamido-2-deoxy-beta-D-glucopyranose, 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHOCHOLINE, ... (4 entities in total)
• 機能のキーワード: abc transporter, membrane protein, translocase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 200594.18

構造登録者

Xie, T.,Zhang, Z.K.,Yue, J.,Gong, X. (登録日: 2021-11-17, 公開日: 2022-04-27, 最終更新日: 2025-06-25)

主引用文献

Xie, T.,Zhang, Z.,Yue, J.,Fang, Q.,Gong, X.
Cryo-EM structures of the human surfactant lipid transporter ABCA3.
Sci Adv, 8:eabn3727-eabn3727, 2022

PubMed Abstract: The adenosine 5'-triphosphate (ATP)-binding cassette (ABC) transporter ABCA3 plays a critical role in pulmonary surfactant biogenesis. Mutations in human ABCA3 have been recognized as the most frequent causes of inherited surfactant dysfunction disorders. Despite two decades of research, in vitro biochemical and structural studies of ABCA3 are still lacking. Here, we report the cryo-EM structures of human ABCA3 in two distinct conformations, both at resolution of 3.3 Å. In the absence of ATP, ABCA3 adopts a "lateral-opening" conformation with the lateral surfaces of transmembrane domains (TMDs) exposed to the membrane and features two positively charged cavities within the TMDs as potential substrate binding sites. ATP binding induces pronounced conformational changes, resulting in the collapse of the potential substrate binding cavities. Our results help to rationalize the disease-causing mutations in human ABCA3 and suggest a conserved "lateral access and extrusion" mechanism for both lipid export and import mediated by ABCA transporters.

PubMed: 35394827
DOI: 10.1126/sciadv.abn3727

実験手法

ELECTRON MICROSCOPY (3.3 Å)