7VY2

STRUCTURE OF PHOTOSYNTHETIC LH1-RC SUPER-COMPLEX OF RHODOBACTER SPHAEROIDES DIMER

これはPDB形式変換不可エントリーです。

7VY2 の概要

• エントリーDOI: 10.2210/pdb7vy2/pdb
• EMDBエントリー: 32192
• 分子名称: Photosynthetic reaction center L subunit, UBIQUINONE-10, DODECYL-BETA-D-MALTOSIDE, ... (17 entities in total)
• 機能のキーワード: lh1-rc complex, photosynthesis, purple bacteria
• 由来する生物種: Rhodobacter sphaeroides f. sp. denitrificans; 詳細
• タンパク質・核酸の鎖数: 66
• 化学式量合計: 702954.78

構造登録者

Tani, K.,Kanno, R.,Kawamura, S.,Kikuchi, R.,Nagashima, K.V.P.,Hall, M.,Takahashi, A.,Yu, L.-J.,Kimura, Y.,Madigan, M.T.,Mizoguchi, A.,Humbel, B.M.,Wang-Otomo, Z.-Y. (登録日: 2021-11-13, 公開日: 2022-04-27, 最終更新日: 2024-10-30)

主引用文献

Tani, K.,Kanno, R.,Kikuchi, R.,Kawamura, S.,Nagashima, K.V.P.,Hall, M.,Takahashi, A.,Yu, L.J.,Kimura, Y.,Madigan, M.T.,Mizoguchi, A.,Humbel, B.M.,Wang-Otomo, Z.Y.
Asymmetric structure of the native Rhodobacter sphaeroides dimeric LH1-RC complex.
Nat Commun, 13:1904-1904, 2022

PubMed Abstract: Rhodobacter sphaeroides is a model organism in bacterial photosynthesis, and its light-harvesting-reaction center (LH1-RC) complex contains both dimeric and monomeric forms. Here we present cryo-EM structures of the native LH1-RC dimer and an LH1-RC monomer lacking protein-U (ΔU). The native dimer reveals several asymmetric features including the arrangement of its two monomeric components, the structural integrity of protein-U, the overall organization of LH1, and rigidities of the proteins and pigments. PufX plays a critical role in connecting the two monomers in a dimer, with one PufX interacting at its N-terminus with another PufX and an LH1 β-polypeptide in the other monomer. One protein-U was only partially resolved in the dimeric structure, signaling different degrees of disorder in the two monomers. The ΔU LH1-RC monomer was half-moon-shaped and contained 11 α- and 10 β-polypeptides, indicating a critical role for protein-U in controlling the number of αβ-subunits required for dimer assembly and stabilization. These features are discussed in relation to membrane topology and an assembly model proposed for the native dimeric complex.

PubMed: 35393413
DOI: 10.1038/s41467-022-29453-8

実験手法

ELECTRON MICROSCOPY (2.75 Å)