7VT2

Azumapecten Farreri ferritin

7VT2 の概要

• エントリーDOI: 10.2210/pdb7vt2/pdb
• 分子名称: Ferritin, FE (III) ION (3 entities in total)
• 機能のキーワード: azumapecten farreri ferritin, iron storage protein, metal binding protein
• 由来する生物種: Azumapecten farreri
• タンパク質・核酸の鎖数: 48
• 化学式量合計: 964265.12

構造登録者

Zhao, G.,Zhang, C. (登録日: 2021-10-27, 公開日: 2022-11-16, 最終更新日: 2023-11-29)

主引用文献

Zhang, C.,Liu, Y.,Zhang, T.,Lv, C.,Zang, J.,Zhao, G.
Structural comparison between the DNA-protective ability of scallop and shrimp ferritin from iron-induced oxidative damage.
Food Chem, 386:132827-132827, 2022

PubMed Abstract: The structure and function of ferritin from seafood has been largely unexplored. In this study, homopolymeric scallop ferritin (ApF) was prepared for the first time, the apo form of which exhibited the stronger ability to protect DNA from iron-induced oxidative damage as compared to its analogue, homopolymeric shrimp ferritin (MjF). Their difference in DNA-protective activity was derived from less hydroxyl radicals produced during iron oxidation in the presence of scallop ferritin than shrimp ferritin. The kinetic results showed that apo-ApF catalyzed the faster ferrous ions oxidation by oxygen into nontoxic ferric ions as compared to apo-MjF. Newly reported crystal structure of ApF revealed that its stronger ferroxidase activity stemmed from different structures in the triple axis channel and ferroxidase site as compared to MjF. All these new findings advance our understanding of the relationship between the structure and function of food-related protein.

PubMed: 35364499
DOI: 10.1016/j.foodchem.2022.132827

実験手法

X-RAY DIFFRACTION (2.4 Å)