7VT1

Acyltransferase from the 9th Module of Salinomycin Polyketide Synthase

Summary for 7VT1

• Entry DOI: 10.2210/pdb7vt1/pdb
• Descriptor: Type I modular polyketide synthase (2 entities in total)
• Functional Keywords: acyl transferase, transferase
• Biological source: Streptomyces albus subsp. albus
• Total number of polymer chains: 2
• Total formula weight: 97146.89

Authors

Feng, Y.,Zhang, F.,Zheng, J. (deposition date: 2021-10-27, release date: 2022-06-08, Last modification date: 2023-11-29)

Primary citation

Feng, Y.,Zhang, F.,Huang, S.,Deng, Z.,Bai, L.,Zheng, J.
Structural visualization of transient interactions between the cis-acting acyltransferase and acyl carrier protein of the salinomycin modular polyketide synthase.
Acta Crystallogr D Struct Biol, 78:779-791, 2022

PubMed Abstract: Transient protein-protein interactions between cis-acting acyltransferase (AT) and acyl carrier protein (ACP) domains are critical for the catalysis and processivity of modular polyketide synthases (mPKSs), but are challenging for structural characterization due to the intrinsically weak binding affinity. Here, a stable complex of cis-acting AT and ACP domains from the ninth module of the salinomycin mPKS was obtained using a maleimide cross-linker and the structure of the complex was determined at 2.6 Å resolution. The crystal structure shows that the AT in combination with the ketosynthase (KS)-to-AT linker forms a C-shaped architecture to embrace the ACP. The large hydrolase subdomain of the AT serves as a major binding platform for the ACP, while the small ferredoxin-like subdomain of the AT and the KS-to-AT linker cooperate with each other to constrain binding of the ACP. The importance of interface residues in cis-acting AT-ACP interactions was confirmed by mutagenesis assays. The interaction mode observed in the cis-acting AT-ACP complex is completely different from those observed in trans-acting AT-ACP complexes, where the ACP primarily contacts the small domain of the AT. The complex structure provides detailed mechanistic insights into AT-ACP recognition in cis-AT mPKSs.

PubMed: 35647924
DOI: 10.1107/S2059798322004612

Experimental method

X-RAY DIFFRACTION (2.5 Å)