7VRL

Solution structure of Rbfox RRM bound to a non-cognate RNA

7VRL の概要

• エントリーDOI: 10.2210/pdb7vrl/pdb
• NMR情報: BMRB: 36452
• 分子名称: RNA (5'-R(*UP*GP*CP*AP*UP*AP*U)-3'), RNA binding protein fox-1 homolog 1 (2 entities in total)
• 機能のキーワード: rrm, non-cognate binding, splicing factor, complex, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 13869.55

構造登録者

Yang, F.,Varani, G. (登録日: 2021-10-23, 公開日: 2022-10-26, 最終更新日: 2024-05-15)

主引用文献

Ye, X.,Yang, W.,Yi, S.,Zhao, Y.,Varani, G.,Jankowsky, E.,Yang, F.
Two distinct binding modes provide the RNA-binding protein RbFox with extraordinary sequence specificity.
Nat Commun, 14:701-701, 2023

PubMed Abstract: Specificity of RNA-binding proteins for target sequences varies considerably. Yet, it is not understood how certain few proteins achieve markedly higher sequence specificity than most others. Here we show that the RNA Recognition Motif of RbFox accomplishes extraordinary sequence specificity by employing functionally and structurally distinct binding modes. Affinity measurements of RbFox for all binding site variants reveal the existence of two distinct binding modes. The first exclusively accommodates cognate and closely related RNAs with high affinity. The second mode accommodates all other RNAs with reduced affinity by imposing large thermodynamic penalties on non-cognate sequences. NMR studies indicate marked structural differences between the two binding modes, including large conformational rearrangements distant from the RNA-binding site. Distinct binding modes by a single RNA-binding module explain extraordinary sequence selectivity and reveal an unknown layer of functional diversity, cross talk and regulation in RNA-protein interactions.

PubMed: 36759600
DOI: 10.1038/s41467-023-36394-3

実験手法

SOLUTION NMR