Summary for 7VQF
| Entry DOI | 10.2210/pdb7vqf/pdb |
| Descriptor | Phenol sensing regulator, MopR, PHENOL, ACETATE ION, ... (5 entities in total) |
| Functional Keywords | transcriptional regulator, phenol, dna binding protein |
| Biological source | Acinetobacter calcoaceticus |
| Total number of polymer chains | 1 |
| Total formula weight | 26631.78 |
| Authors | |
| Primary citation | Singh, J.,Sahil, M.,Ray, S.,Dcosta, C.,Panjikar, S.,Krishnamoorthy, G.,Mondal, J.,Anand, R. Phenol sensing in nature is modulated via a conformational switch governed by dynamic allostery. J.Biol.Chem., 298:102399-102399, 2022 Cited by PubMed Abstract: The NtrC family of proteins senses external stimuli and accordingly stimulates stress and virulence pathways via activation of associated σ-dependent RNA polymerases. However, the structural determinants that mediate this activation are not well understood. Here, we establish using computational, structural, biochemical, and biophysical studies that MopR, an NtrC protein, harbors a dynamic bidirectional electrostatic network that connects the phenol pocket to two distal regions, namely the "G-hinge" and the "allosteric linker." While the G-hinge influences the entry of phenol into the pocket, the allosteric linker passes the signal to the downstream ATPase domain. We show that phenol binding induces a rewiring of the electrostatic connections by eliciting dynamic allostery and demonstrates that perturbation of the core relay residues results in a complete loss of ATPase stimulation. Furthermore, we found a mutation of the G-hinge, ∼20 Å from the phenol pocket, promotes altered flexibility by shifting the pattern of conformational states accessed, leading to a protein with 7-fold enhanced phenol binding ability and enhanced transcriptional activation. Finally, we conducted a global analysis that illustrates that dynamic allostery-driven conserved community networks are universal and evolutionarily conserved across species. Taken together, these results provide insights into the mechanisms of dynamic allostery-mediated conformational changes in NtrC sensor proteins. PubMed: 35988639DOI: 10.1016/j.jbc.2022.102399 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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