7VPY

Crystal structure of the neutralizing nanobody P86 against SARS-CoV-2

7VPY の概要

• エントリーDOI: 10.2210/pdb7vpy/pdb
• 分子名称: Nanobody, SULFATE ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: sars-cov-2, immune system
• 由来する生物種: Vicugna pacos
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 32216.01

構造登録者

Maeda, R.,Fujita, J.,Konishi, Y.,Kazuma, Y.,Yamazaki, H.,Anzai, I.,Yamaguchi, K.,Kasai, K.,Nagata, K.,Yamaoka, Y.,Miyakawa, K.,Ryo, A.,Shirakawa, K.,Makino, F.,Matsuura, Y.,Inoue, T.,Imura, A.,Namba, K.,Takaori-Kondo, A. (登録日: 2021-10-18, 公開日: 2022-07-20, 最終更新日: 2024-10-30)

主引用文献

Maeda, R.,Fujita, J.,Konishi, Y.,Kazuma, Y.,Yamazaki, H.,Anzai, I.,Watanabe, T.,Yamaguchi, K.,Kasai, K.,Nagata, K.,Yamaoka, Y.,Miyakawa, K.,Ryo, A.,Shirakawa, K.,Sato, K.,Makino, F.,Matsuura, Y.,Inoue, T.,Imura, A.,Namba, K.,Takaori-Kondo, A.
A panel of nanobodies recognizing conserved hidden clefts of all SARS-CoV-2 spike variants including Omicron.
Commun Biol, 5:669-669, 2022

PubMed Abstract: We are amid the historic coronavirus infectious disease 2019 (COVID-19) pandemic. Imbalances in the accessibility of vaccines, medicines, and diagnostics among countries, regions, and populations, and those in war crises, have been problematic. Nanobodies are small, stable, customizable, and inexpensive to produce. Herein, we present a panel of nanobodies that can detect the spike proteins of five SARS-CoV-2 variants of concern (VOCs) including Omicron. Here we show via ELISA, lateral flow, kinetic, flow cytometric, microscopy, and Western blotting assays that our nanobodies can quantify the spike variants. This panel of nanobodies broadly neutralizes viral infection caused by pseudotyped and authentic SARS-CoV-2 VOCs. Structural analyses show that the P86 clone targets epitopes that are conserved yet unclassified on the receptor-binding domain (RBD) and contacts the N-terminal domain (NTD). Human antibodies rarely access both regions; consequently, the clone buries hidden crevasses of SARS-CoV-2 spike proteins that go undetected by conventional antibodies.

PubMed: 35794202
DOI: 10.1038/s42003-022-03630-3

実験手法

X-RAY DIFFRACTION (1.6 Å)