7VPD

Cryo-EM structure of Streptomyces coelicolor RNAP-promoter open complex with one Zur dimers

7VPD の概要

• エントリーDOI: 10.2210/pdb7vpd/pdb
• EMDBエントリー: 32063
• 分子名称: DNA-directed RNA polymerase subunit alpha, ZINC ION, DNA-directed RNA polymerase subunit beta, ... (10 entities in total)
• 機能のキーワード: dna binding protein
• 由来する生物種: Streptomyces coelicolor A3(2); 詳細
• タンパク質・核酸の鎖数: 11
• 化学式量合計: 538949.34

構造登録者

Yang, X.,Zheng, J. (登録日: 2021-10-15, 公開日: 2022-08-03, 最終更新日: 2024-06-19)

主引用文献

Yang, X.,Wang, Y.,Liu, G.,Deng, Z.,Lin, S.,Zheng, J.
Structural basis of Streptomyces transcription activation by zinc uptake regulator.
Nucleic Acids Res., 50:8363-8376, 2022

PubMed Abstract: Streptomyces coelicolor (Sc) is a model organism of actinobacteria to study morphological differentiation and production of bioactive metabolites. Sc zinc uptake regulator (Zur) affects both processes by controlling zinc homeostasis. It activates transcription by binding to palindromic Zur-box sequences upstream of -35 elements. Here we deciphered the molecular mechanism by which ScZur interacts with promoter DNA and Sc RNA polymerase (RNAP) by cryo-EM structures and biochemical assays. The ScZur-DNA structures reveal a sequential and cooperative binding of three ScZur dimers surrounding a Zur-box spaced 8 nt upstream from a -35 element. The ScRNAPσHrdB-Zur-DNA structures define protein-protein and protein-DNA interactions involved in the principal housekeeping σHrdB-dependent transcription initiation from a noncanonical promoter with a -10 element lacking the critical adenine residue at position -11 and a TTGCCC -35 element deviating from the canonical TTGACA motif. ScZur interacts with the C-terminal domain of ScRNAP α subunit (αCTD) in a complex structure trapped in an active conformation. Key ScZur-αCTD interfacial residues accounting for ScZur-dependent transcription activation were confirmed by mutational studies. Together, our structural and biochemical results provide a comprehensive model for transcription activation of Zur family regulators.

PubMed: 35871291
DOI: 10.1093/nar/gkac627

実験手法

ELECTRON MICROSCOPY (3.77 Å)