7VNL

Sandercyanin mutant-F55A-Biliverdin complex

7VNL の概要

• エントリーDOI: 10.2210/pdb7vnl/pdb
• 分子名称: Sandercyanin Fluorescent Protein, BILIVERDINE IX ALPHA, TETRAETHYLENE GLYCOL, ... (4 entities in total)
• 機能のキーワード: lipocalin, biliverdin, fluorescent protein
• 由来する生物種: Sander vitreus (walleye)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41721.05

構造登録者

Yadav, K.,Ghosh, S.,Subramanian, R. (登録日: 2021-10-11, 公開日: 2022-05-25, 最終更新日: 2024-10-30)

主引用文献

Yadav, K.,Ghosh, S.,Barak, A.,Schaefer, W.,Subramanian, R.
Phenylalanine stacking enhances the red fluorescence of biliverdin IX alpha on UV excitation in sandercyanin fluorescent protein.
Febs Lett., 596:796-805, 2022

PubMed Abstract: Biliverdin IXα (BV) binds to several prokaryotic and eukaryotic proteins. How nature exploits the versatility of BV's properties is not fully understood. Unlike free BV, the Sandercyanin fluorescent protein bound to BV (SFP-BV) shows enhanced red fluorescence (675 nm) on excitation in the UV region (380 nm). Site-directed mutagenesis showed that the BV complex of two SFP variants, F55A and E79A, resulted in the loss of red fluorescence. Crystal structures of the complexes of these proteins with BV show the absence of stacking interactions of the F55 phenyl ring with BV. BV changes from ZZZssa conformation in the wild-type to ZZZsss conformation in the variants. In the nonfluorescent mutants, the lowest excited state is destabilized, resulting in nonradiative decay.

PubMed: 35020202
DOI: 10.1002/1873-3468.14281

実験手法

X-RAY DIFFRACTION (1.93 Å)