7VN3

Crystal structure of MBP-fused BIL1/BZR1 (21-90) in complex with double-stranded DNA contaning CACACGTGTG

7VN3 の概要

• エントリーDOI: 10.2210/pdb7vn3/pdb
• 関連するPDBエントリー: 5ZD4
• 関連するBIRD辞書のPRD_ID: PRD_900001
• 分子名称: Maltodextrin-binding protein,Protein BRASSINAZOLE-RESISTANT 1, DNA (5'-D(*TP*TP*CP*AP*CP*AP*CP*GP*TP*GP*TP*GP*AP*AP*A)-3'), alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose, ... (5 entities in total)
• 機能のキーワード: transcription factor-dna complex, transcription
• 由来する生物種: Serratia sp. (strain FS14); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 213855.05

構造登録者

Nosaki, S.,Tanokura, M.,Miyakawa, T. (登録日: 2021-10-10, 公開日: 2022-12-07, 最終更新日: 2023-11-29)

主引用文献

Nosaki, S.,Mitsuda, N.,Sakamoto, S.,Kusubayashi, K.,Yamagami, A.,Xu, Y.,Bui, T.B.C.,Terada, T.,Miura, K.,Nakano, T.,Tanokura, M.,Miyakawa, T.
Brassinosteroid-induced gene repression requires specific and tight promoter binding of BIL1/BZR1 via DNA shape readout.
Nat.Plants, 8:1440-1452, 2022

PubMed Abstract: BRZ-INSENSITIVE-LONG 1 (BIL1)/BRASSINAZOLE-RESISTANT 1 (BZR1) and its homologues are plant-specific transcription factors that convert the signalling of the phytohormones brassinosteroids (BRs) to transcriptional responses, thus controlling various physiological processes in plants. Although BIL1/BZR1 upregulates some BR-responsive genes and downregulates others, the molecular mechanism underlying the dual roles of BIL1/BZR1 is still poorly understood. Here we show that BR-responsive transcriptional repression by BIL1/BZR1 requires the tight binding of BIL1/BZR1 alone to the 10 bp elements of DNA fragments containing the known 6 bp core-binding motifs at the centre. Furthermore, biochemical and structural evidence demonstrates that the selectivity for two nucleobases flanking the core motifs is realized by the DNA shape readout of BIL1/BZR1 without direct recognition of the nucleobases. These results elucidate the molecular and structural basis of transcriptional repression by BIL1/BZR1 and contribute to further understanding of the dual roles of BIL1/BZR1 in BR-responsive gene regulation.

PubMed: 36522451
DOI: 10.1038/s41477-022-01289-6

実験手法

X-RAY DIFFRACTION (1.94 Å)