7VMD
Crystal structure of a hydrolases Ple628 from marine microbial consortium
Summary for 7VMD
Entry DOI | 10.2210/pdb7vmd/pdb |
Descriptor | hydrolase Ple628, CALCIUM ION (3 entities in total) |
Functional Keywords | degradation, hydrolase |
Biological source | unclassified Marinobacter |
Total number of polymer chains | 1 |
Total formula weight | 31308.28 |
Authors | |
Primary citation | Meyer Cifuentes, I.E.,Wu, P.,Zhao, Y.,Liu, W.,Neumann-Schaal, M.,Pfaff, L.,Barys, J.,Li, Z.,Gao, J.,Han, X.,Bornscheuer, U.T.,Wei, R.,Ozturk, B. Molecular and Biochemical Differences of the Tandem and Cold-Adapted PET Hydrolases Ple628 and Ple629, Isolated From a Marine Microbial Consortium. Front Bioeng Biotechnol, 10:930140-930140, 2022 Cited by PubMed Abstract: Polybutylene adipate terephthalate (PBAT) is a biodegradable alternative to polyethylene and can be broadly used in various applications. These polymers can be degraded by hydrolases of terrestrial and aquatic origin. In a previous study, we identified tandem PETase-like hydrolases (Ples) from the marine microbial consortium I1 that were highly expressed when a PBAT blend was supplied as the only carbon source. In this study, the tandem Ples, Ple628 and Ple629, were recombinantly expressed and characterized. Both enzymes are mesophilic and active on a wide range of oligomers. The activities of the Ples differed greatly when model substrates, PBAT-modified polymers or PET nanoparticles were supplied. Ple629 was always more active than Ple628. Crystal structures of Ple628 and Ple629 revealed a structural similarity to other PETases and can be classified as member of the PETases IIa subclass, α/β hydrolase superfamily. Our results show that the predicted functions of Ple628 and Ple629 agree with the bioinformatic predictions, and these enzymes play a significant role in the plastic degradation by the consortium. PubMed: 35935485DOI: 10.3389/fbioe.2022.930140 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (1.69 Å) |
Structure validation
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