7VM9

Solution NMR structures of DNA minidumbbell formed with two regular CTTTG pentaloops

Summary for 7VM9

• Entry DOI: 10.2210/pdb7vm9/pdb
• Descriptor: DNA (5'-D(*CP*TP*TP*TP*GP*CP*TP*TP*TP*G)-3'), SODIUM ION (2 entities in total)
• Functional Keywords: dna, dna structure, minidumbbell, ctttg pentaloop
• Biological source: synthetic construct
• Total number of polymer chains: 1
• Total formula weight: 3223.88

Authors

Ngai, C.K.,Guo, P. (deposition date: 2021-10-08, release date: 2022-02-16, Last modification date: 2024-05-15)

Primary citation

Ngai, C.K.,Lam, S.L.,Lee, H.K.,Guo, P.
A purine and a backbone discontinuous site alter the structure and thermal stability of DNA minidumbbells containing two pentaloops.
Febs Lett., 596:826-840, 2022

PubMed Abstract: Minidumbbell (MDB) is a noncanonical DNA structure found to form in several pyrimidine-rich short tandem repeats associated with neurodegenerative diseases. The most recently reported MDB contains two pentaloops formed by ATTCT repeats. Here, we studied the effects of a purine residue and a backbone discontinuous site on the structure and thermal stability of MDBs containing two pentaloops. It was found that a purine as the fourth loop residue improved the thermal stability of MDBs containing two regular pentaloops, while a backbone discontinuous site between the third and fourth, or between the fourth and fifth loop residues enhanced the thermal stability of MDBs containing a regular and a quasi pentaloops. The results of this study provide new insights into the sequence criteria and structural basis of MDBs.

PubMed: 35060128
DOI: 10.1002/1873-3468.14293

Experimental method

SOLUTION NMR