7VHQ

Structural insights into the membrane microdomain organization by SPFH family proteins

7VHQ の概要

• エントリーDOI: 10.2210/pdb7vhq/pdb
• EMDBエントリー: 32003
• 分子名称: ATP-dependent zinc metalloprotease FtsH, Protein HflK, Modulator of FtsH protease HflC (3 entities in total)
• 機能のキーワード: membrane microdomain organization, membrane protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 246092.87

構造登録者

Ma, C.Y.,Wang, C.K.,Luo, D.Y.,Yan, L.,Yang, W.X.,Li, N.N.,Gao, N. (登録日: 2021-09-22, 公開日: 2022-01-19, 最終更新日: 2025-06-25)

主引用文献

Ma, C.,Wang, C.,Luo, D.,Yan, L.,Yang, W.,Li, N.,Gao, N.
Structural insights into the membrane microdomain organization by SPFH family proteins.
Cell Res., 32:176-189, 2022

PubMed Abstract: The lateral segregation of membrane constituents into functional microdomains, conceptually known as lipid raft, is a universal organization principle for cellular membranes in both prokaryotes and eukaryotes. The widespread Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH) family proteins are enriched in functional membrane microdomains at various subcellular locations, and therefore were hypothesized to play a scaffolding role in microdomain formation. In addition, many SPFH proteins are also implicated in highly specific processes occurring on the membrane. However, none of these functions is understood at the molecular level. Here we report the structure of a supramolecular complex that is isolated from bacterial membrane microdomains and contains two SPFH proteins (HflK and HflC) and a membrane-anchored AAA+ protease FtsH. HflK and HflC form a circular 24-mer assembly, featuring a laterally segregated membrane microdomain (20 nm in diameter) bordered by transmembrane domains of HflK/C and a completely sealed periplasmic vault. Four FtsH hexamers are embedded inside this microdomain through interactions with the inner surface of the vault. These observations provide a mechanistic explanation for the role of HflK/C and their mitochondrial homologs prohibitins in regulating membrane-bound AAA+ proteases, and suggest a general model for the organization and functionalization of membrane microdomains by SPFH proteins.

PubMed: 34975153
DOI: 10.1038/s41422-021-00598-3

実験手法

ELECTRON MICROSCOPY (3.27 Å)