7VEB

Phycocyanin rod structure of cyanobacterial phycobilisome

7VEB の概要

• エントリーDOI: 10.2210/pdb7veb/pdb
• EMDBエントリー: 31945
• 分子名称: C-phycocyanin alpha subunit, C-phycocyanin beta subunit, Phycobilisome 8.9 kDa linker polypeptide, phycocyanin-associated, rod, ... (6 entities in total)
• 機能のキーワード: phycobilisome, phycocyanin rod, light-harvesting, photosynthesis
• 由来する生物種: Thermosynechococcus elongatus BP-1; 詳細
• タンパク質・核酸の鎖数: 27
• 化学式量合計: 518930.55

構造登録者

Kawakami, K.,Hamaguchi, T.,Hirose, Y.,Kosumi, D.,Miyata, M.,Kamiya, N.,Yonekura, K. (登録日: 2021-09-08, 公開日: 2022-06-15, 最終更新日: 2022-08-17)

主引用文献

Kawakami, K.,Hamaguchi, T.,Hirose, Y.,Kosumi, D.,Miyata, M.,Kamiya, N.,Yonekura, K.
Core and rod structures of a thermophilic cyanobacterial light-harvesting phycobilisome.
Nat Commun, 13:3389-3389, 2022

PubMed Abstract: Cyanobacteria, glaucophytes, and rhodophytes utilize giant, light-harvesting phycobilisomes (PBSs) for capturing solar energy and conveying it to photosynthetic reaction centers. PBSs are compositionally and structurally diverse, and exceedingly complex, all of which pose a challenge for a comprehensive understanding of their function. To date, three detailed architectures of PBSs by cryo-electron microscopy (cryo-EM) have been described: a hemiellipsoidal type, a block-type from rhodophytes, and a cyanobacterial hemidiscoidal-type. Here, we report cryo-EM structures of a pentacylindrical allophycocyanin core and phycocyanin-containing rod of a thermophilic cyanobacterial hemidiscoidal PBS. The structures define the spatial arrangement of protein subunits and chromophores, crucial for deciphering the energy transfer mechanism. They reveal how the pentacylindrical core is formed, identify key interactions between linker proteins and the bilin chromophores, and indicate pathways for unidirectional energy transfer.

PubMed: 35715389
DOI: 10.1038/s41467-022-30962-9

実験手法

ELECTRON MICROSCOPY (4.2 Å)