7VDY

Crystal structure of O-ureidoserine racemase

7VDY の概要

• エントリーDOI: 10.2210/pdb7vdy/pdb
• 分子名称: O-ureido-serine racemase, SULFATE ION (3 entities in total)
• 機能のキーワード: racemase, isomerase
• 由来する生物種: Streptomyces lavendulae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63943.75

構造登録者

Oda, K.,Matoba, Y. (登録日: 2021-09-07, 公開日: 2021-12-15, 最終更新日: 2023-11-29)

主引用文献

Oda, K.,Sakaguchi, T.,Matoba, Y.
Crystal structure of O-ureidoserine racemase found in the d-cycloserine biosynthetic pathway.
Proteins, 90:912-918, 2022

PubMed Abstract: The O-ureidoserine racemase (DcsC) is an enzyme found from the biosynthetic gene cluster of antitubercular agent d-cycloserine. Although DcsC is homologous to diaminopimelate epimerase (DapF) that catalyzes the interconversion between ll- and dl-diaminopimelic acid, it specifically catalyzes the interconversion between O-ureido-l-serine and its enantiomer. Here we determined the crystal structure of DcsC at a resolution of 2.12 Å, implicating that the catalytic mechanism of DcsC shares similarity with that of DapF. Comparing the structure of the active center of DcsC to that of DapF, Thr72, Thr198, and Tyr219 of DcsC are likely to be involved in the substrate specificity.

PubMed: 34877716
DOI: 10.1002/prot.26290

実験手法

X-RAY DIFFRACTION (2.12 Å)