7VCM

crystal structure of GINKO1

Summary for 7VCM

• Entry DOI: 10.2210/pdb7vcm/pdb
• Descriptor: Green fluorescent protein,Potassium binding protein Kbp,Green fluorescent protein, POTASSIUM ION (3 entities in total)
• Functional Keywords: green fluorescent protein, genetically encoded biosensor, potassium binding domain protein, fluorescent protein
• Biological source: Aequorea victoria (Water jellyfish, Mesonema victoria); More
• Total number of polymer chains: 2
• Total formula weight: 86105.23

Authors

Wen, Y.,Campbell, R.E.,Lemieux, M.J. (deposition date: 2021-09-03, release date: 2022-07-27, Last modification date: 2024-11-06)

Primary citation

Wu, S.Y.,Wen, Y.,Serre, N.B.C.,Laursen, C.C.H.,Dietz, A.G.,Taylor, B.R.,Drobizhev, M.,Molina, R.S.,Aggarwal, A.,Rancic, V.,Becker, M.,Ballanyi, K.,Podgorski, K.,Hirase, H.,Nedergaard, M.,Fendrych, M.,Lemieux, M.J.,Eberl, D.F.,Kay, A.R.,Campbell, R.E.,Shen, Y.
A sensitive and specific genetically-encoded potassium ion biosensor for in vivo applications across the tree of life.
Plos Biol., 20:e3001772-e3001772, 2022

PubMed Abstract: Potassium ion (K+) plays a critical role as an essential electrolyte in all biological systems. Genetically-encoded fluorescent K+ biosensors are promising tools to further improve our understanding of K+-dependent processes under normal and pathological conditions. Here, we report the crystal structure of a previously reported genetically-encoded fluorescent K+ biosensor, GINKO1, in the K+-bound state. Using structure-guided optimization and directed evolution, we have engineered an improved K+ biosensor, designated GINKO2, with higher sensitivity and specificity. We have demonstrated the utility of GINKO2 for in vivo detection and imaging of K+ dynamics in multiple model organisms, including bacteria, plants, and mice.

PubMed: 36067248
DOI: 10.1371/journal.pbio.3001772

Experimental method

X-RAY DIFFRACTION (1.85 Å)