7VBA の概要
| エントリーDOI | 10.2210/pdb7vba/pdb |
| EMDBエントリー | 31876 |
| 分子名称 | DNA-directed RNA polymerase I subunit RPA1, DNA-directed RNA polymerases I, II, and III subunit RPABC4, DNA-directed RNA polymerase I subunit RPA34, ... (19 entities in total) |
| 機能のキーワード | rna polymerase i, transcription, pre, state, transcription-dna-rna complex, transcription/dna/rna |
| 由来する生物種 | Homo sapiens (Human) 詳細 |
| タンパク質・核酸の鎖数 | 16 |
| 化学式量合計 | 616625.71 |
| 構造登録者 | |
| 主引用文献 | Zhao, D.,Liu, W.,Chen, K.,Wu, Z.,Yang, H.,Xu, Y. Structure of the human RNA polymerase I elongation complex. Cell Discov, 7:97-97, 2021 Cited by PubMed Abstract: Eukaryotic RNA polymerase I (Pol I) transcribes ribosomal DNA and generates RNA for ribosome synthesis. Pol I accounts for the majority of cellular transcription activity and dysregulation of Pol I transcription leads to cancers and ribosomopathies. Despite extensive structural studies of yeast Pol I, structure of human Pol I remains unsolved. Here we determined the structures of the human Pol I in the pre-translocation, post-translocation, and backtracked states at near-atomic resolution. The single-subunit peripheral stalk lacks contacts with the DNA-binding clamp and is more flexible than the two-subunit stalk in yeast Pol I. Compared to yeast Pol I, human Pol I possesses a more closed clamp, which makes more contacts with DNA. The Pol I structure in the post-cleavage backtracked state shows that the C-terminal zinc ribbon of RPA12 inserts into an open funnel and facilitates "dinucleotide cleavage" on mismatched DNA-RNA hybrid. Critical disease-associated mutations are mapped on Pol I regions that are involved in catalysis and complex organization. In summary, the structures provide new sights into human Pol I complex organization and efficient proofreading. PubMed: 34671025DOI: 10.1038/s41421-021-00335-5 主引用文献が同じPDBエントリー |
| 実験手法 | ELECTRON MICROSCOPY (2.89 Å) |
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