7VB8

SbSOMT in complex with resveratrol and nicotinamide adenine dinucleotide(NAD+)

7VB8 の概要

• エントリーDOI: 10.2210/pdb7vb8/pdb
• 関連するPDBエントリー: 7WAQ 7WAR 7WAS
• 分子名称: stilbene O-methyltransferase, RESVERATROL, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (8 entities in total)
• 機能のキーワード: resveratrol, complex, o-methyltransferase, substrate, transferase
• 由来する生物種: Sorghum bicolor (sorghum)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 86553.41

構造登録者

Pow, K.C.,Hao, Q. (登録日: 2021-08-30, 公開日: 2022-09-07, 最終更新日: 2024-04-03)

主引用文献

Lui, A.C.W.,Pow, K.C.,Lin, N.,Lam, L.P.Y.,Liu, G.,Godwin, I.D.,Fan, Z.,Khoo, C.J.,Tobimatsu, Y.,Wang, L.,Hao, Q.,Lo, C.
Regioselective stilbene O-methylations in Saccharinae grasses.
Nat Commun, 14:3462-3462, 2023

PubMed Abstract: O-Methylated stilbenes are prominent nutraceuticals but rarely produced by crops. Here, the inherent ability of two Saccharinae grasses to produce regioselectively O-methylated stilbenes is reported. A stilbene O-methyltransferase, SbSOMT, is first shown to be indispensable for pathogen-inducible pterostilbene (3,5-bis-O-methylated) biosynthesis in sorghum (Sorghum bicolor). Phylogenetic analysis indicates the recruitment of genus-specific SOMTs from canonical caffeic acid O-methyltransferases (COMTs) after the divergence of Sorghum spp. from Saccharum spp. In recombinant enzyme assays, SbSOMT and COMTs regioselectively catalyze O-methylation of stilbene A-ring and B-ring respectively. Subsequently, SOMT-stilbene crystal structures are presented. Whilst SbSOMT shows global structural resemblance to SbCOMT, molecular characterizations illustrate two hydrophobic residues (Ile144/Phe337) crucial for substrate binding orientation leading to 3,5-bis-O-methylations in the A-ring. In contrast, the equivalent residues (Asn128/Asn323) in SbCOMT facilitate an opposite orientation that favors 3'-O-methylation in the B-ring. Consistently, a highly-conserved COMT is likely involved in isorhapontigenin (3'-O-methylated) formation in wounded wild sugarcane (Saccharum spontaneum). Altogether, our work reveals the potential of Saccharinae grasses as a source of O-methylated stilbenes, and rationalize the regioselectivity of SOMT activities for bioengineering of O-methylated stilbenes.

PubMed: 37308495
DOI: 10.1038/s41467-023-38908-5

実験手法

X-RAY DIFFRACTION (1.72 Å)