7V6D

Structure of lipase B from Lasiodiplodia theobromae

7V6D の概要

• エントリーDOI: 10.2210/pdb7v6d/pdb
• 分子名称: Lipase B, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: alpha/beta-hydrolases, hydrolase
• 由来する生物種: Lasiodiplodia theobromae
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 90658.13

構造登録者

Xue, B.,Zhang, H.F.,Nguyen, G.K.T.,Yew, W.S. (登録日: 2021-08-20, 公開日: 2021-10-13, 最終更新日: 2023-11-29)

主引用文献

Ng, A.M.J.,Yang, R.,Zhang, H.,Xue, B.,Yew, W.S.,Nguyen, G.K.T.
A Novel Lipase from Lasiodiplodia theobromae Efficiently Hydrolyses C8-C10 Methyl Esters for the Preparation of Medium-Chain Triglycerides' Precursors.
Int J Mol Sci, 22:-, 2021

PubMed Abstract: Medium-chain triglycerides (MCTs) are an emerging choice to treat neurodegenerative disorders such as Alzheimer's disease. They are triesters of glycerol and three medium-chain fatty acids, such as capric (C8) and caprylic (C10) acids. The availability of C8-C10 methyl esters (C8-C10 ME) from vegetable oil processes has presented an opportunity to use methyl esters as raw materials for the synthesis of MCTs. However, there are few reports on enzymes that can efficiently hydrolyse C8-C10 ME to industrial specifications. Here, we report the discovery and identification of a novel lipase from fungus (LTL1), which hydrolyses C8-C10 ME efficiently. LTL1 can perform hydrolysis over pH ranges from 3.0 to 9.0 and maintain thermotolerance up to 70 °C. It has high selectivity for monoesters over triesters and displays higher activity over commercially available lipases for C8-C10 ME to achieve 96.17% hydrolysis within 31 h. Structural analysis by protein X-ray crystallography revealed LTL1's well-conserved lipase core domain, together with a partially resolved N-terminal subdomain and an inserted loop, which may suggest its hydrolytic preference for monoesters. In conclusion, our results suggest that LTL1 provides a tractable route towards to production of C8-C10 fatty acids from methyl esters for the synthesis of MCTs.

PubMed: 34638680
DOI: 10.3390/ijms221910339

実験手法

X-RAY DIFFRACTION (2.5 Å)