7V02

Staphylococcus epidermidis RP62A CRISPR short effector complex

7V02 の概要

• エントリーDOI: 10.2210/pdb7v02/pdb
• EMDBエントリー: 26927
• 分子名称: CRISPR system Cms endoribonuclease Csm3, CRISPR system Cms protein Csm5, CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A), ... (6 entities in total)
• 機能のキーワード: type iiia crispr, effector complex, rna binding protein, hydrolase-rna complex, hydrolase/rna
• 由来する生物種: Staphylococcus epidermidis RP62A; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 276557.53

構造登録者

Smith, E.M.,Ferrell, S.H.,Tokars, V.L.,Mondragon, A. (登録日: 2022-05-09, 公開日: 2022-07-06, 最終更新日: 2025-08-20)

主引用文献

Smith, E.M.,Ferrell, S.,Tokars, V.L.,Mondragon, A.
Structures of an active type III-A CRISPR effector complex.
Structure, 30:1109-1128.e6, 2022

PubMed Abstract: Clustered regularly interspaced short palindromic repeats (CRISPR) and their CRISPR-associated proteins (Cas) provide many prokaryotes with an adaptive immune system against invading genetic material. Type III CRISPR systems are unique in that they can degrade both RNA and DNA. In response to invading nucleic acids, they produce cyclic oligoadenylates that act as secondary messengers, activating cellular nucleases that aid in the immune response. Here, we present seven single-particle cryo-EM structures of the type III-A Staphylococcus epidermidis CRISPR effector complex. The structures reveal the intact S. epidermidis effector complex in an apo, ATP-bound, cognate target RNA-bound, and non-cognate target RNA-bound states and illustrate how the effector complex binds and presents crRNA. The complexes bound to target RNA capture the type III-A effector complex in a post-RNA cleavage state. The ATP-bound structures give details about how ATP binds to Cas10 to facilitate cyclic oligoadenylate production.

PubMed: 35714601
DOI: 10.1016/j.str.2022.05.013

実験手法

ELECTRON MICROSCOPY (4.97 Å)