7UXF

Cryogenic electron microscopy 3D map of F-actin

7UXF の概要

• エントリーDOI: 10.2210/pdb7uxf/pdb
• EMDBエントリー: 26772 26860
• 分子名称: Actin, alpha skeletal muscle, ADENOSINE-5'-DIPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: f-actin, cell-cell junction, cell adhesion
• 由来する生物種: Oryctolagus cuniculus (rabbit)
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 255368.87

構造登録者

Rangarajan, E.S.,Smith, E.W.,Izard, T. (登録日: 2022-05-05, 公開日: 2023-03-08, 最終更新日: 2023-03-29)

主引用文献

Rangarajan, E.S.,Smith, E.W.,Izard, T.
Distinct inter-domain interactions of dimeric versus monomeric alpha-catenin link cell junctions to filaments.
Commun Biol, 6:276-276, 2023

PubMed Abstract: Attachment between cells is crucial for almost all aspects of the life of cells. These inter-cell adhesions are mediated by the binding of transmembrane cadherin receptors of one cell to cadherins of a neighboring cell. Inside the cell, cadherin binds β-catenin, which interacts with α-catenin. The transitioning of cells between migration and adhesion is modulated by α-catenin, which links cell junctions and the plasma membrane to the actin cytoskeleton. At cell junctions, a single β-catenin/α-catenin heterodimer slips along filamentous actin in the direction of cytoskeletal tension which unfolds clustered heterodimers to form catch bonds with F-actin. Outside cell junctions, α-catenin dimerizes and links the plasma membrane to F-actin. Under cytoskeletal tension, α-catenin unfolds and forms an asymmetric catch bond with F-actin. To understand the mechanism of this important α-catenin function, we determined the 2.7 Å cryogenic electron microscopy (cryoEM) structures of filamentous actin alone and bound to human dimeric α-catenin. Our structures provide mechanistic insights into the role of the α-catenin interdomain interactions in directing α-catenin function and suggest a bivalent mechanism. Further, our cryoEM structure of human monomeric α-catenin provides mechanistic insights into α-catenin autoinhibition. Collectively, our structures capture the initial α-catenin interaction with F-actin before the sensing of force, which is a crucial event in cell adhesion and human disease.

PubMed: 36928388
DOI: 10.1038/s42003-023-04610-x

実験手法

ELECTRON MICROSCOPY (2.7 Å)