7UWE
CryoEM Structure of E. coli Transcription-Coupled Ribonucleotide Excision Repair (TC-RER) complex
Summary for 7UWE
| Entry DOI | 10.2210/pdb7uwe/pdb |
| EMDB information | 26830 26832 |
| Descriptor | DNA (29-MER), ZINC ION, RNA (18-MER), ... (10 entities in total) |
| Functional Keywords | transcription-coupled rer, transcription, transferase-hydrolase-dna-rna complex, transferase/hydrolase/dna/rna |
| Biological source | Escherichia coli More |
| Total number of polymer chains | 9 |
| Total formula weight | 434781.62 |
| Authors | Hao, Z.T.,Grower, M.,Bharati, B.,Proshkin, S.,Epshtein, V.,Svetlov, V.,Nudler, E.,Shamovsky, I. (deposition date: 2022-05-03, release date: 2023-05-31, Last modification date: 2024-06-12) |
| Primary citation | Hao, Z.,Gowder, M.,Proshkin, S.,Bharati, B.K.,Epshtein, V.,Svetlov, V.,Shamovsky, I.,Nudler, E. RNA polymerase drives ribonucleotide excision DNA repair in E. coli. Cell, 186:2425-, 2023 Cited by PubMed Abstract: Ribonuclease HII (RNaseHII) is the principal enzyme that removes misincorporated ribonucleoside monophosphates (rNMPs) from genomic DNA. Here, we present structural, biochemical, and genetic evidence demonstrating that ribonucleotide excision repair (RER) is directly coupled to transcription. Affinity pull-downs and mass-spectrometry-assisted mapping of in cellulo inter-protein cross-linking reveal the majority of RNaseHII molecules interacting with RNA polymerase (RNAP) in E. coli. Cryoelectron microscopy structures of RNaseHII bound to RNAP during elongation, with and without the target rNMP substrate, show specific protein-protein interactions that define the transcription-coupled RER (TC-RER) complex in engaged and unengaged states. The weakening of RNAP-RNaseHII interactions compromises RER in vivo. The structure-functional data support a model where RNaseHII scans DNA in one dimension in search for rNMPs while "riding" the RNAP. We further demonstrate that TC-RER accounts for a significant fraction of repair events, thereby establishing RNAP as a surveillance "vehicle" for detecting the most frequently occurring replication errors. PubMed: 37196657DOI: 10.1016/j.cell.2023.04.029 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (2.9 Å) |
Structure validation
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