7UW5

EcMscK G924S mutant in a closed conformation

7UW5 の概要

• エントリーDOI: 10.2210/pdb7uw5/pdb
• EMDBエントリー: 26823
• 分子名称: Mechanosensitive channel MscK (1 entity in total)
• 機能のキーワード: transport protein, membrane protein, mechanosensation, ion channel
• 由来する生物種: Escherichia coli K-12
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 891651.52

構造登録者

Mount, J.W.,Yuan, P. (登録日: 2022-05-02, 公開日: 2022-11-23, 最終更新日: 2024-06-12)

主引用文献

Mount, J.,Maksaev, G.,Summers, B.T.,Fitzpatrick, J.A.J.,Yuan, P.
Structural basis for mechanotransduction in a potassium-dependent mechanosensitive ion channel.
Nat Commun, 13:6904-6904, 2022

PubMed Abstract: Mechanosensitive channels of small conductance, found in many living organisms, open under elevated membrane tension and thus play crucial roles in biological response to mechanical stress. Amongst these channels, MscK is unique in that its activation also requires external potassium ions. To better understand this dual gating mechanism by force and ligand, we elucidate distinct structures of MscK along the gating cycle using cryo-electron microscopy. The heptameric channel comprises three layers: a cytoplasmic domain, a periplasmic gating ring, and a markedly curved transmembrane domain that flattens and expands upon channel opening, which is accompanied by dilation of the periplasmic ring. Furthermore, our results support a potentially unifying mechanotransduction mechanism in ion channels depicted as flattening and expansion of the transmembrane domain.

PubMed: 36371466
DOI: 10.1038/s41467-022-34737-0

実験手法

ELECTRON MICROSCOPY (3.84 Å)