7UUQ

Cryo-EM of self-assembling pyrene IDP

7UUQ の概要

• エントリーDOI: 10.2210/pdb7uuq/pdb
• EMDBエントリー: 26800
• 分子名称: Pyrene-containing peptide fibril (1 entity in total)
• 機能のキーワード: peptides, nanofibers, self-assembly peptide filament, protein fibril
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 7420.05

構造登録者

Wang, F.,Guo, J.,Xu, B.,Egelman, E.H. (登録日: 2022-04-28, 公開日: 2023-05-10, 最終更新日: 2025-05-21)

主引用文献

Guo, J.,Rich-New, S.T.,Liu, C.,Huang, Y.,Tan, W.,He, H.,Yi, M.,Zhang, X.,Egelman, E.H.,Wang, F.,Xu, B.
Hierarchical Assembly of Intrinsically Disordered Short Peptides.
Chem, 9:2530-2546, 2023

PubMed Abstract: The understanding on how short peptide assemblies transit from disorder to order remains limited due to the lack of atomistic structures. Here we report cryo-EM structure of the nanofibers short intrinsically disordered peptides (IDPs). Upon lowering pH or adding calcium ions, the IDP transitions from individual nanoparticles to nanofibers containing an aromatic core and a disordered periphery comprised of 2 to 5 amino acids. Protonating the phosphate or adding more metal ions further assembles the nanofibers into filament bundles. The assemblies of the IDP analogs with controlled chemistry, such as phosphorylation site, hydrophobic interactions, and sequences indicate that metal ions interact with the flexible periphery of the nanoparticles of the IDPs to form fibrils and enhance the interfibrillar interactions to form filament bundles. Illustrating that an IDP self-assembles from disorder to order, this work offers atomistic molecular insights to understand assemblies of short peptides driven by noncovalent interactions.

PubMed: 38094164
DOI: 10.1016/j.chempr.2023.04.023

実験手法

ELECTRON MICROSCOPY (3.1 Å)