7UTU

CPV Total-Fab Polyclonal B Site Fab (1 of 2)

これはPDB形式変換不可エントリーです。

7UTU の概要

• エントリーDOI: 10.2210/pdb7utu/pdb
• EMDBエントリー: 26789
• 分子名称: Light chain antibody fragment, Heavy chain antibody fragment, Capsid protein 2 (3 entities in total)
• 機能のキーワード: cpv, polyclonal fab, b site, vaccination, virus, virus-immune system complex, virus/immune system
• 由来する生物種: Canis lupus familiaris; 詳細
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 510406.99

構造登録者

Hartmann, S.R.,Hafenstein, S.L.,Charnesky, A.J. (登録日: 2022-04-27, 公開日: 2023-10-04, 最終更新日: 2024-10-30)

主引用文献

Hartmann, S.R.,Charnesky, A.J.,Fruh, S.P.,Lopez-Astacio, R.A.,Weichert, W.S.,DiNunno, N.,Cho, S.H.,Bator, C.M.,Parrish, C.R.,Hafenstein, S.L.
Cryo EM structures map a post vaccination polyclonal antibody response to canine parvovirus.
Commun Biol, 6:955-955, 2023

PubMed Abstract: Canine parvovirus (CPV) is an important pathogen that emerged by cross-species transmission to cause severe disease in dogs. To understand the host immune response to vaccination, sera from dogs immunized with parvovirus are obtained, the polyclonal antibodies are purified and used to solve the high resolution cryo EM structures of the polyclonal Fab-virus complexes. We use a custom software, Icosahedral Subparticle Extraction and Correlated Classification (ISECC) to perform subparticle analysis and reconstruct polyclonal Fab-virus complexes from two different dogs eight and twelve weeks post vaccination. In the resulting polyclonal Fab-virus complexes there are a total of five distinct Fabs identified. In both cases, any of the five antibodies identified would interfere with receptor binding. This polyclonal mapping approach identifies a specific, limited immune response to the live vaccine virus and allows us to investigate the binding of multiple different antibodies or ligands to virus capsids.

PubMed: 37726539
DOI: 10.1038/s42003-023-05319-7

実験手法

ELECTRON MICROSCOPY (3 Å)