7US9

CCoV-HuPn-2018 S in the proximal conformation (local refinement of domain 0)

Summary for 7US9

• Entry DOI: 10.2210/pdb7us9/pdb
• EMDB information: 26729
• Descriptor: Spike glycoprotein, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose (3 entities in total)
• Functional Keywords: human coronavirus, coronavirus, ccov-hupn-2018, spike glycoprotein, alpha-coronaviruses, structural genomics, seattle structural genomics center for infectious disease, ssgcid, viral protein
• Biological source: unidentified human coronavirus
• Total number of polymer chains: 1
• Total formula weight: 30826.86

Authors

Tortorici, M.A.,Veesler, D.,Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2022-04-23, release date: 2022-08-24, Last modification date: 2024-10-30)

Primary citation

Tortorici, M.A.,Walls, A.C.,Joshi, A.,Park, Y.J.,Eguia, R.T.,Miranda, M.C.,Kepl, E.,Dosey, A.,Stevens-Ayers, T.,Boeckh, M.J.,Telenti, A.,Lanzavecchia, A.,King, N.P.,Corti, D.,Bloom, J.D.,Veesler, D.
Structure, receptor recognition, and antigenicity of the human coronavirus CCoV-HuPn-2018 spike glycoprotein.
Cell, 185:2279-2291.e17, 2022

PubMed Abstract: The isolation of CCoV-HuPn-2018 from a child respiratory swab indicates that more coronaviruses are spilling over to humans than previously appreciated. We determined the structures of the CCoV-HuPn-2018 spike glycoprotein trimer in two distinct conformational states and showed that its domain 0 recognizes sialosides. We identified that the CCoV-HuPn-2018 spike binds canine, feline, and porcine aminopeptidase N (APN) orthologs, which serve as entry receptors, and determined the structure of the receptor-binding B domain in complex with canine APN. The introduction of an oligosaccharide at position N739 of human APN renders cells susceptible to CCoV-HuPn-2018 spike-mediated entry, suggesting that single-nucleotide polymorphisms might account for viral detection in some individuals. Human polyclonal plasma antibodies elicited by HCoV-229E infection and a porcine coronavirus monoclonal antibody inhibit CCoV-HuPn-2018 spike-mediated entry, underscoring the cross-neutralizing activity among ɑ-coronaviruses. These data pave the way for vaccine and therapeutic development targeting this zoonotic pathogen representing the eighth human-infecting coronavirus.

PubMed: 35700730
DOI: 10.1016/j.cell.2022.05.019

Experimental method

ELECTRON MICROSCOPY (3.8 Å)