7URN

Structure of HIV-1 capsid declination

7URN の概要

• エントリーDOI: 10.2210/pdb7urn/pdb
• EMDBエントリー: 26715
• 分子名称: HIV-1 capsid protein, INOSITOL HEXAKISPHOSPHATE (2 entities in total)
• 機能のキーワード: hiv-1, capsid, declination, pentamer, hexamer, virus like particle
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 180733.05

構造登録者

Pornillos, O.,Ganser-Pornillos, B.K.,Schirra, R.T. (登録日: 2022-04-22, 公開日: 2023-02-15, 最終更新日: 2024-05-15)

主引用文献

Schirra, R.T.,Dos Santos, N.F.B.,Zadrozny, K.K.,Kucharska, I.,Ganser-Pornillos, B.K.,Pornillos, O.
A molecular switch modulates assembly and host factor binding of the HIV-1 capsid.
Nat.Struct.Mol.Biol., 30:383-390, 2023

PubMed Abstract: The HIV-1 capsid is a fullerene cone made of quasi-equivalent hexamers and pentamers of the viral CA protein. Typically, quasi-equivalent assembly of viral capsid subunits is controlled by a molecular switch. Here, we identify a Thr-Val-Gly-Gly motif that modulates CA hexamer/pentamer switching by folding into a 3 helix in the pentamer and random coil in the hexamer. Manipulating the coil/helix configuration of the motif allowed us to control pentamer and hexamer formation in a predictable manner, thus proving its function as a molecular switch. Importantly, the switch also remodels the common binding site for host factors that are critical for viral replication and the new ultra-potent HIV-1 inhibitor lenacapavir. This study reveals that a critical assembly element also modulates the post-assembly and viral replication functions of the HIV-1 capsid and provides new insights on capsid function and inhibition.

PubMed: 36759579
DOI: 10.1038/s41594-022-00913-5

実験手法

ELECTRON MICROSCOPY (3.43 Å)